胶束电动毛细管电泳同时检测刺五加中紫丁香苷及绿原酸的含量

 目的建立简单、快速且能同时分离测定刺五加中紫丁香苷、绿原酸含量的胶束电动毛细管电泳法。方法采用胶束电动毛细管电泳法,缓冲体系为100mmol·L^-1 NaH₂PO₄-100mmol·L^-1 Borax-100mmol·L^-1 SDS-双蒸水(6.25∶43.75∶37.5∶12.5,pH9.25),熔融石英毛细管柱(25μm×45cm),分离电压为16kV,检测波长为254nm,进样时间为25s,进样高度10cm。结果紫丁香苷、绿原酸在0.0625～2.0000,0.2500～8.0000g·L^-1与峰面积线性关系良好,线性方程依次为:Y=112904ρ+1645.1(r=0.9999),Y=98191ρ-165.11(r=0.9999);样品回收率分别为100.78%和99.56%;两种成分含量以刺五加根中最高。结论该方法简单、快速、准确、重现性好,可用于刺五加药材的质量控制,不同部位两成分均存在一定的差异。

Simultaneous Determination of Syringin and Chlorogenic Acid in Acanthopanax senticosus ( Rupr. et Maxim) by Micellar Electrokinetic Capillary Chromatography

OBJECTIVE To develop a simple and rapid Micellar Electrokinetic Capillary Chromatography coupled with UV detection for the determination of syringin and chlorogenic acid in Acanthopanax senticosus (Rupr. et Maxim) simultaneously. METHODS The buffer consisted of 100 mmol·L^-1 NaH₂PO₄,100 mmol·L^-1 Borax,100 mmol·L^-1 SDS and double distilled water (6.25∶43.75∶37.5∶12.5,pH 9.25). Capillary electrophoresis was performed using a 25 μm×45 cm fused silica capillary column. Separation voltage was 16 kV,detection wavelength was set at 254 nm,and the injection time was 25 s at a height of 10 cm.RESULTS The linear range of syringin was from 0.062 5 to 2.000 0 g·L^-1,linear regression equation was Y=112 904ρ+1 645.6 (r=0.999 9),and the average recovery was 100.78%,while the linear range of chlorogenic acid was from 0.250 0 to 8.000 0 g·L^-1,linear regression equation was Y=98 191ρ-165.11 (r=0.999 9),and the average recovery was 99.56%. Their contents were highest in roots.CONCLUSION This method can be used for the quality control of the Acanthopanax senticosus (Rupr. et Maxim) with good precision. The contents of syringin and chlorogenic acid are different in Acanthopanax senticosus (Rupr. et Maxim) from different parts.