拆方研究通降和胃方对胃食管反流性咳嗽豚鼠气道炎症及肺通透性的影响

目的]观察通降和胃方及其拆方对胃食管反流性咳嗽豚鼠模型气道炎症及肺组织通透性的影响,探讨通降和胃方治疗胃食管反流性咳嗽的作用机制。方法]健康雄性豚鼠60只,随机分为通降和胃方组、疏肝泄热拆方组、和胃降逆拆方组、西药组、模型组和正常组。除正常组外,其余各组采用食管下端酸灌注法制作胃食管反流性咳嗽模型,并分别予相应药物混悬液及等量生理盐水处理,光镜下观察食管及气管组织学改变,伊文思蓝浸入法测定各组豚鼠的肺毛细血管通透性。结果]1)模型组食管、气管炎症积分较正常组明显升高;疏肝泄热拆方组、和胃降逆拆方组在改善豚鼠气管炎症上存在交互作用(P0.05);通降和胃方与奥美拉唑在改善豚鼠食管、气管炎症积分上比较差异均有统计学意义(P0.05)。2)模型组肺组织通透性较正常组明显升高(P0.05),通降和胃方与奥美拉唑在改善豚鼠肺组织通透性上比较差异有统计学意义(P0.05),疏肝泄热拆方组、和胃降逆拆方组在改善肺组织通透性上存在交互作用(P0.05)。结论]通降和胃方全方能有效改善豚鼠食管、气管组织学改变,降低肺组织通透性,从而减少炎性渗出,达到治疗胃食管反流性咳嗽的目的。

Effect of decomposed recipes of Tongjiang Hewei decoction on guinea pigs with gastroesophageal reflux induced cough

Objective] To observe the effect of the Tongjiang Hewei decoction on airway inflammation and pulmonary tissue permeability in guinea pig model of gastroesophageal reflux induced cough. Methods] Sixty healthy male guinea pigs were randomly divided into 6 groups:Tongjiang Hewei group, Shugan Xiere group, Hewei Jiangni group, control group, model group and normal group. GERC models were induced repeatedly by hydrochloric acid instillation, except normal group, given the corresponding intervention measures to each group. After two weeks, execute all animals and observe the histological changes of the esophagus and trachea; evans Blue-invasive method for the determination of pulmonary capillary permeability in guinea pigs. Results] 1)The inflammation of esophagus and trachea in model group was much more obviously than the normal group; the interaction between Shugan Xiere group and Hewei Jiangni group was significant in improving the guinea pig esophagus and trachea tissue inflammation. Comparing Tongjiang Hewei decoction with omeprazole, there was a significant difference in improving the guinea pig esophagus and trachea pathologic scores (P<0.05). 2) Permeability of the model lung tissue than normal group was significantly increased respectively(P<0.05); the interaction between Shugan Xiere group and Hewei Jiangni group was significant in improving the permeability(P<0.05); Tongjiang Hewei decoction and omeprazole had a significant difference in this area(P<0.05). Conclusion] Tongjiang Hewei decoction can effectively improve the guinea pig esophagus and trachea inflammation, reduce the permeability of the lung tissue, thereby reduce the inflammatory exudate and cure cough.