Survivin反义寡核苷酸联合AsI3-cys对K562细胞凋亡的影响

目的:探讨Survivin反义寡核苷酸(ASODN)联合AsI3-cys对K562细胞增殖的抑制作用和细胞凋亡的诱导作用。方法:K562细胞体外培养,人工合成并硫代修饰Survivin ASODN,通过脂质体转染进入K562细胞,同时与AsI3-cys联用,MTT法检测细胞增殖抑制程度,Tunel法检测细胞凋亡指数,RT-PCR检测细胞中Survivin mRNA的表达水平,流式细胞仪检测细胞周期的变化和细胞凋亡率。结果:Survivin ASODN各浓度组对K562细胞的增殖有明显的抑制作用,能明显诱导细胞凋亡,联用组诱导K562细胞凋亡的能力与其他组相比在统计学上差异有显著性。结论:Survivin ASODN能够诱导K562细胞凋亡,并提高K562细胞对AsI3-cys的敏感性。

Survivin antisense ollgodeoxy-nucleotid and AsI3-cys induce apoptosis of K562 cells

Objective To investigate the effect of survivin antisense oligodeoxy-nucleotid(ASODN) and AsI3-cys on cell proliferation and cell apoptosis of K562 cells. Methods K562 cells were serially subcultured. Survivin ASODN was transfected into K562 cells by lipofectamine mediated transient transfection method. Cell proliferation was measured by MTT method and cell apoptosis index was detected by Tunel,RT-PCR was used to detect survivin gene expression,FCM was used to detect apoptosis rate and cell cycle. Results Survivin ASODN had obvious inhibitory effects on the cell proliferation and induced cell apoptosis of K562 cells. The cell apoptosis rate in Survivin ASODN and AsI3-cys group was obviously higher than three control groups. Conclusion Survivin ASODN can induce cell apoptosis and enhance AsI3-cys induced apoptosis in K562 cells.