缝隙连接蛋白Cx43在大鼠经脉线上表达的实验研究

目的:研究缝隙连接蛋白Cx43在正常健康大鼠经脉线上的表达及其意义.方法:应用二步法免疫酶技术和ASIAS-2000自动扫描图像分析系统对大鼠多条经脉上的Cx43进行定位、定量分析.结果:缝隙连接蛋白Cx43广泛表达于表皮上皮细胞、真皮成纤维细胞、皮下筋膜的肥大细胞以及肌肉肌膜细胞的胞浆胞膜中,经统计学分析,肾经足底段、膀胱经背部段Cx43的表达显著高于邻近对照线,两者相比差异有极显著性意义(P＜0.01).结论:缝隙连接蛋白及缝隙连接与经络高度相关,由缝隙连接介导的细胞间通讯可能在经络活动中起重要作用.

Experimental study on expression of connexin 43 in meridians of rats

Objective To probe into expression of connexin 43 (Cx43) in the meridians of the normal healthy rats and the relation among connexin, gap junction and meridian. Methods PowerVision TM two step immunohistochemical technique and ASIAS-2000 automatical image-scan analyzing system were used to detect Cx43 level and distribution in the Kidney and Bladder Meridians of the rat. Results Cx43 expressed mainly in skin epithelia, fibroblasts and mast cells of the subcutaneous layer. And expression of Cx43 in the Kidney and Bladder Meridians was significantly more than that in the control lines (P<0.01). Conclusion Connexins and gap junctions have close relation with the meridians, and the gap junctional intercellular communication may play an important role in the function of meridians.