绝经后健康妇女雌激素受体基因多态性与骨密度关系的研究

目的从分子生物学水平探讨中国绝经后健康妇女雌激素受体（ＥＲ）基因多态性与骨密度的关系。方法选择绝经后健康、无亲缘关系妇女２３７例，运用双能Ｘ线吸收骨密度仪及聚合酶链反应——限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）方法分析ＥＲ基因多态性与骨密度的关系。结果２３７例绝经后健康妇女中ＰＰ、Ｐｐ及ｐｐ基因型频率分别为０．１９８，０．４４３及０．３５９；Ｐ与ｐ等位基因频率分别为０．４１９８、０．５８０２。方差分析（ＡＮＯＶＡ）显示股骨大转子部位骨密度与ＥＲ基因多态性相关（ｐ＝０．０１０６），ＰＰ基因型各部位骨密度值均低于Ｐｐ、ｐｐ基因型。逐步多元回归分析发现ＥＲ基因多态性与股骨大转子（ｐ＝０．０５４８）及腰椎２－４（Ｐ＝０．０９９８）部位的骨密度相关。结论中国绝经后健康妇女ＥＲ基因多态性与股骨大转子部位的骨密度显著相关，ｐ等位基因具有一定的骨量保护作用，提示从分子水平探讨骨量丢失规律以及防治骨质疏松症的发生发展具有一定的现实意义。

Relationship between dietary habits and bone density of women in Beijing

The relationship between bone density of radius and dietary habits was examined in a sample of 434 healthy women aged 20 65 years,selected from five urban communities in Beijing.Questionnaire was used to survey the dietary habits in the past 2 years.The bone density of radius was measured by a single photon absorptiometer The data of multiple regression analysis showed that the BMD was positively related to the consumption of dark green vegetables and dairy products.The resules of this study suggest that consumpion of dark green uegetables and dairy products has beneficial effects on the prevention of bone loss of women.