己酮可可碱对大鼠急性高眼压性视网膜缺血再灌注损伤的保护作用

目的:探讨己酮可可碱对大鼠视网膜缺血再灌注损伤的保护作用及机制。方法:视网膜缺血再灌注模型是通过提高前房眼内压来实现的。将35只Wistar大鼠随机分为3组:正常组5只,对照组15只,治疗组15只。治疗组于高眼压前6h和眼压正常后即刻己酮可可碱50mg/kgip,对照组于相同时间点等容量生理盐水ip。监测视网膜电流图(ERG)b波的变化,测定视网膜谷氨酸含量以及视网膜线粒体钙含量。结果:治疗组和对照组谷氨酸含量都在缺血再灌注后逐渐上升,在48h达到峰值,两组都明显高于空白对照组(P<0.01)。在6h点治疗组与对照组间无显著差异,在其他时点对照组的Glu含量明显高于治疗组(P<0.05)。治疗组和对照组视网膜线粒体钙含量在缺血再灌注后逐渐上升,在24h达到峰值,然后逐渐下降。两组都明显高于空白对照组(P<0.01)。在6h点治疗组与对照组间无显著差异,在其他时点对照组的视网膜线粒体钙浓度明显高于治疗组(P<0.05)。再灌注后,对照组ERGb波比较低平;再灌注后1h,对照组、治疗组的ERG相对b波无明显的差异。24h后两组b波都降低到最低,然后逐渐恢复;72h后对照组ERGb波仅恢复到正常眼的69%左右。己酮可可碱处理组ERGb波恢复到正常眼的95%左右,ERGb波明显较对照组高(P<0.01)。结论:己酮可可碱能有效降低视网膜谷氨酸含量及视网膜组织细胞线粒体钙离子含量,促进ERGb波的恢复,缩短了视网膜缺血再灌注的病理过程。对大鼠视网膜缺血再灌注损伤有一定的保护作用。

Protective effects and mechanism of Pentoxifylline against retinal ischemia/reperfusion injury in Wistar rats

AIM:To observe the protective effects of Pentoxifylline against retinal ischemia/reperfusion injury in Wistar rats,and to investigate its mechanism. METHODS:A total of 35 Wistar rats were randomly divided into three groups as sham(5),control(15)and treatment(15)groups.After application of high in-traocular pressure for 60 minutes for the induction of retinal ischemia,72-hour reperfusion was established in the control and treatment groups.In the control,saline was given and in treatment groups,50mg/kg of Pen...