| 卡介苗胞壁蛋白诱导THP-1细胞IL-12mRNA表达 |
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| 引用本文： | 唐琼,孔祥丽,杨云霞,冯云,吴琦,王伯瑶. 卡介苗胞壁蛋白诱导THP-1细胞IL-12mRNA表达[J]. 四川生理科学杂志, 2003, 25(1): 24-26 |
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| 作者姓名： | 唐琼 孔祥丽 杨云霞 冯云 吴琦 王伯瑶 |
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| 作者单位： | 四川大学华西医学中心基础与法医学院感染免疫研究室,成都,610041;四川大学华西医学中心基础与法医学院药理教研室,成都,610041;四川大学华西医学中心基础与法医学院感染免疫研究室,成都,610041;四川大学华西医学中心基础与法医学院感染免疫研究室,成都,610041;四川大学华西医学中心基础与法医学院,成都,610041 |
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| 摘 要： | 目的：研究卡介苗(BCG)能否诱导人单核白细胞系细胞THP—1、IL-l2 P40基因表达,并鉴定其胞壁蛋白的活性组份。方法：应用逆转录聚合酶链反应(RT—PCR)法检测THP—1细胞IL-l2 P40mRNA的表达;采用超声破碎细胞、蔗糖密度梯度离心、Sephadex G—150柱层析等方法分离卡介苗胞壁蛋白质组份。结果：卡介苗胞壁蛋白Sephadex G—150柱层析所得组分中,分子量范围约21—29kD的组份诱导THP—1细胞IL-12 P40mRNA的表达增强。结论：结果显示BCG胞壁蛋白可刺激白细胞IL-l2 P40基因的表达。
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| 关 键 词： | THP-1细胞 IL-12 基因表达 卡介苗 胞壁蛋白 |
Enhancement of IL-12 mRNA expression in THP-1 cells induced by BCG cell wall protein |
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| Tang Qiong ,,Kong Xiangli,Yang Yunsia ,Feng yun ,Wu qi ,Wang Boyao. Enhancement of IL-12 mRNA expression in THP-1 cells induced by BCG cell wall protein[J]. Sichuan Journal of Physiological Sciences, 2003, 25(1): 24-26 |
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| Authors: | Tang Qiong Kong Xiangli Yang Yunsia Feng yun Wu qi Wang Boyao |
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| Affiliation: | Tang Qiong 1,2,Kong Xiangli,Yang Yunsia 2,Feng yun 1,Wu qi 1,Wang Boyao 1 |
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| Abstract: | Abstact Objection: To study the BCG cell wall proteins that stimulate IL 12 gene expression in human THP 1 cells. Methods: Using reverse transcription PCR (RT PCR) , IL 12 mRNA expression in THP 1 cells was detected. Results: The induced expression of IL 12p40 mRNA was dose and time dependent. SDS PAGE showed its molecular range of a 21 29KD. Conclusion: These results provided that BCG cell wall proteins could stimulate IL 12p40 mRNA expression in human THP 1 cells. |
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| Keywords: | THP 1 cell IL 12 Gene expression BCG Cell wall proteins |
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