环氧化酶在膀胱移行细胞癌中的表达

目的　了解环氧化酶 (COX)在膀胱移行细胞癌中的表达及其与肿瘤分期和分级的关系。方法　对34例开放手术切除的膀胱癌组织标本及对照组 7例肾移植供体正常膀胱组织标本运用 COX- 1及 COX- 2的单克隆抗体进行免疫组织化学染色检测表达情况 ;并运用 RT- PCR对 COX- 1及 COX- 2的 m RNA表达进行检测。结果正常膀胱中未见 COX- 2表达 ,而在膀胱移行细胞癌细胞中均有 COX- 2表达。在移行细胞癌组织中 COX- 2的表达强度随肿瘤分级程度的增加呈现升高趋势 (P<0 .0 0 1)。肿瘤临床各期的 COX- 2免疫组织化学染色程度未见明显统计学差异 (P值均 >0 .0 5 )。COX- 1在膀胱移行细胞癌和正常膀胱中均有表达。结论　环氧化酶 - 2在膀胱移行细胞癌中异常表达 ,该异常表达可能与膀胱移行细胞癌的发生发展有关。

Expression of Cyclooxygenase in Bladder Transitional Cell Carcinoma

Objective To evaluate the role of cyclooxygenase-1(COX-1) and cyclooxygenase-2 (COX-2) in the development of bladder transitional cell carcinoma. Methods Thirty-four specimens were obtained from patients with bladder transitional carcinoma, and 7 specimens of normal bladder tissues were obtained from renal transplantation donors. Immunohistochemistry and RT-PCR were performed to study the expression of COX-1 and COX-2. Results No marked expression of COX-2 was observed in the normal bladder, but higher level of COX-2 expression was noted in the malignant cells. The extent and intensity of COX-2 expression in cancer cells were statistically greater than those in the cells from normal bladder tissue. Correlation between COX-2 expression and progression of bladder transitional cell carcinoma was observed. COX-2 expression was significantly higher in Grade 3 bladder transitional cell carcinoma than in Grades 1 and 2 (P<0.001). There was no significant difference in COX-2 expression level between the bladder transitional cell carcinomas at different clinical stages (P>0.05). The expression of COX-1 was seen in some bladder transitional cell carcinoma and normal bladder. Conclusion COX-2 might be of significance to the proliferation and development of bladder transitional cell carcinoma.