非侵入性聚焦超声辐照活猪皮下脂肪层即刻结果的组织学观察

目的:观察聚焦超声照射活体实验用小型猪皮下脂肪后的组织学即刻表现。方法:采用频率1.9MHz,焦域不大于12mm,平均声功率(550±20%)W的聚焦超声照射猪背部皮下脂肪,即刻切取包括皮肤全层与肌肉浅层的标本块制作病理切片,HE染色,组织学观察。结果:镜下可见脂肪层内存在大量点状脂肪细胞破坏损伤灶。损伤灶有10个细胞大小,灶内脂肪细胞破裂、消失,多个损伤灶可融合成较大损伤区。照射区与损伤灶内均未见出血与游离红细胞,未见皮肤和肌层损伤。结论:脉冲输出的聚焦超声可选择性破坏脂肪细胞。

Instant histological observation of fat irradiated by non-invasive focused ultrasound

Objective To observe instant histological change of Pig subcutaneous fat layer what irradiated by non-invasive focused ultrasound.Methods Pig subcutaneous fat layer was irradiated by non-invasive focused ultrasound with frequency 1.9MHz,focal field was not bigger than 12mm,the average sound power(550±20%) W,cut full-thickness skin and superficial muscle biopsy specimen to make pathological section,HE staining and histological observation.Results A large number of point-like fat cell damage focuses were in the fat layer under microscope,the damage focuses were 10 cells size,the fat cells inside were broken down,disappeared,and a number of damage focuses were integrate into a larger damage zone.free red blood cells,skin and muscle damage were not seen in both irradiation zone and damage focus.Conclusion The focused ultrasound of pulse output can selectively destroy fat cells.