| Effects of electroacupuncture combined with hydrogel on the formation and changes in the glial scar in rats with spinal cord injuryOA |
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| 引用本文： | Kaitan Yang,Yushan Gao,Yongdong Yang,Chuanhong Li,Zheng Cao,Xiumei Wang,Zaibo Li,Xing Yu. Effects of electroacupuncture combined with hydrogel on the formation and changes in the glial scar in rats with spinal cord injuryOA[J]. 中医科学杂志(英文), 2022, 9(2): 197-205. DOI: 10.1016/j.jtcms.2022.04.002 |
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| 作者姓名： | Kaitan Yang Yushan Gao Yongdong Yang Chuanhong Li Zheng Cao Xiumei Wang Zaibo Li Xing Yu |
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| 作者单位： | 1. Ward Three, Department of Orthopedics, Beijing University of Chinese Medicine Dongzhimen Hospital;2. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine;3. School of Materials Science and Engineering, Tsinghua University;4. Department of Pathology, The OHIO State University Wexner Medical Center |
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| 基金项目： | financially supported by the National Natural Science Foundation of China (81973882 and 81804119)； |
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| 摘 要： | Objective: To observe the effect of electroacupuncture(EA) combined with oriented conductive bioprotein hydrogel(OCBH) on the recovery of nerve function in rats with complete spinal cord injury(SCI)and to explore its effect and mechanism on the formation and changes of glial scars.Methods: A total of 72 female Sprague-Dawley rats were randomly divided into groups according to the treatment received. A rat model of complete SCI was constructed using a spinal cord transection.Behavioral assessment...
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| 收稿时间： | 2022-02-21 |
Effects of electroacupuncture combined with hydrogel on the formation and changes in the glial scar in rats with spinal cord injury |
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| Affiliation: | 1. Ward Three, Department of Orthopedics, Beijing University of Chinese Medicine Dongzhimen Hospital, 100010, Beijing, China;2. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China;3. School of Materials Science and Engineering, Tsinghua University, 100084, Beijing, China;4. Department of Pathology, The OHIO State University Wexner Medical Center, Columbus, OH, 43210, USA |
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| Abstract: | ObjectiveTo observe the effect of electroacupuncture (EA) combined with oriented conductive bioprotein hydrogel (OCBH) on the recovery of nerve function in rats with complete spinal cord injury (SCI) and to explore its effect and mechanism on the formation and changes of glial scars.MethodsA total of 72 female Sprague–Dawley rats were randomly divided into groups according to the treatment received. A rat model of complete SCI was constructed using a spinal cord transection. Behavioral assessments, hematoxylin-eosin (H&E) staining, immunofluorescence staining, and Western blotting were performed at a fixed period after the operation.ResultsThe material group and the material + EA group obtained better results in the behavioral assessments (all P < .05) and the H&E staining. In the immunofluorescence staining and Western blotting, the GFAP protein was expressed more and denser in the material group and the material + EA group than in the model group, and the density of the GFAP expression in the material + EA group was lower at week 12 than in the material group (all P < .05). The expression of complement C3 in the model, material, and material + EA groups decreased in turn. Some inflammatory factors and the NF-κB signaling pathway showed similar results in the Western blotting (all P < .05). The expression of the GDNF protein in the material + EA group was significantly higher than that in the model group and the material group (both P < .01).ConclusionEA combined with OCBH can promote the recovery of motor functions after SCI by facilitating the formation of glial scars in the early stage, preventing the further spread of an inflammatory response that would affect the activation of A1/A2 astrocytes and change the morphology of glial scars at the spinal cord–material interface in its late stage. |
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| Keywords: | Spinal cord injury Glial scar Astrocytes Electroacupuncture Hydrogel |
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