布鲁氏菌A19疫苗株全基因组测序及比较基因组学分析

目的 探索布鲁氏杆菌A19疫苗株全基因组的结构、分子生物学的功能,并对其生物信息学进行研究。方法 采用Illumina Hiseq 4000和PacBio对A19进行全基因组测序,并与GenBank 上的8株菌进行比较基因组学解析。A19基因组3 286 167 bp, 预测3 371个基因,GC含量57.25%。通过注释COG库,对应基因有2 560个,将其归入22类COG中;根据比对KEGG库,得到2 544个基因,共参与33类代谢通路。结果 综合两个数据库结果发现,大多数A19预测基因中的基因功能主要与膜运输、氨基酸转运及碳水化合物代谢有关。结论 通过分析发现, A19和猪羊牛种布鲁氏菌之间存在一定差异,并找出牛种毒力基因。本实验通过测序A19全基因组,为布鲁菌疫苗的研究提供思路。

Whole-genome sequencing and comparative genomics analysis of Brucella vaccine strain A19

This study explores the structure of whole genome, molecular biology functions and bioinformatics analysis of the Brucella vaccine strain A19. Whole genome sequencing of A19 was performed using Illumina Hiseq 4000 and PacBio sequencing technology, then comparative genomics analysis were carried out with 8 strains of Brucella released in GenBank. The A19 genome is 3 286 167 bp, and the predicted genes was 3 371 with a GC value of 57.25%. Through the COG database annotation, 2 560 genes were corresponding to it and classified into 22 COGs; through the KEGG database, 2 544 genes were obtained, which involved 33 types of metabolic pathways. Combining the results from two databases, most of the gene functions in A19 predictive genes are related to membrane transport, amino acid transport and carbohydrate metabolism. In summary, there was a certain difference among the Brucella vaccine strain A19 and other Brucella strains. Also some virulence genes of Brucella abortus were found. This study provides ideas for further study of Brucella vaccine by performing the whole genome sequencing of A19.