乳鼠肝细胞的体外培养及组织化学观察

本文用等量混合低浓度胶原酶、胰蛋白酶,直接消化乳鼠肝组织小块的方法,获得了存活率较高的大量肝细胞。培养的乳鼠肝细胞呈上皮状,形态多样,可分为大形细胞和小形细胞,胞质内充满颗粒。在培养的肝细胞内糖原丰富,琥珀酸脱氢酶活性强,乳酸脱氢酶活性阳性。培养的乳鼠肝细胞具有整体肝中细胞的特殊功能,可做为研究肝细胞的实验模型。

Culture and Histochemical Observation of Hepatocytes in Baby Rats

In this paper, low concentration and equal quantum of collagenase and trypsin were mixed and used to digest directly small piece of liver tissue of baby rats. Large amount of vital cells were obtained by using this method. Cultured hepatocytes are epithelioid cells and have various shape. The cells can be classified large ones and small ones. In the cytoplasm, large amount of granulations were observed, were observed. With histochemical observation, many glucogen granulation can be demonstrated. In hepatocytes, the activity of SDH and LDH is high. Cultured hepatocytes have the function of hepatocytes in whole liver and can be used as experimental mould in studying physiological function,differentiation and cancer research of hepatocyte.