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Residence time distributions of solutes in the perfused rat liver using a dispersion model of hepatic elimination: 1. Effect of changes in perfusate flow and albumin concentration on sucrose and taurocholate |
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| Authors: | Michael S. Roberts Sharon Fraser Andrew Wagner Lyndsay McLeod |
| Affiliation: | (1) Department of Pharmacy, Otago Medical School, Dunedin, New Zealand;(2) Present address: Departments of Pharmacy and Medicine, University of Queensland, 4067 St. Lucia, Queensland, Australia;(3) School of Pharmacy, University of Tasmania, Hobart, Australia;(4) Department of Physiology, University of Tasmania, Hobart, Australia |
| Abstract: | The residence time distributions of sucrose and taurocholate have been determined from the outflow concentration-time profiles after bolus input into an in situperfused rat liver preparation. The normalized variance (and the dispersion number) appeared to be independent of perfusate flow rate (10 to 37ml/mm) and perfusate albumin concentration (0–5%). The apparent volume of distribution for sucrose appeared to increase with flow rate but was unaffected by the concentration of albumin (0–5%) present in the perfusate. The changes in taurocholate availability with flow rate were adequately accounted for by the dispersion model, whereas taurocholate availabilityprotein binding changes required an albumin-mediated transport model to be used in conjunction with the dispersion model.This study was supported by the National Health and Medical Research Council of Australia and the Dean's MRC (NZ) Fund. |
| Keywords: | liver metabolism hepatic models residence time distribution dispersion model perfusate flow albumin concentration rat liver |
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