推拉动作对脑组织一氧化氮、一氧化氮合酶及血浆内皮素的影响

为研究推拉动作对脑组织一氧化氮 (NO)、一氧化氮合酶 (NOS)及血浆内皮素 (ET)的影响 ,探讨推拉动作时在较低的G值即可发生加速度引起的意识丧失(G inducedlessofconsciousness,G LOC)的机制 ,给予大鼠高正加速度 ( Gz)及推拉动作暴露 ,并于暴露后30min、3h、1 2h、2 4h和 4 8h 5个时间点麻醉采血、取脑 ,测定脑组织NO、NOS及血浆中ET的含量。结果发现 , Gz和推拉动作组与正常组比较 ,脑组织中的NO、NOS及血浆中ET的含量在 30min、3h和 1 2h时增多 ,差异有显著性意义 (P <0 0 1 ) ,2 4h和 4 8h组间比较差异无显著性意义 (P >0 0 5 ) ;推拉动作组与 Gz暴露组比较 ,30min、3h和 1 2h 3个时相点脑组织中的NO、NOS及血浆中ET的含量差异有显著性意义 (P <0 0 5 )。提示推拉动作暴露和单纯高 Gz暴露可使大鼠脑组织NO与血浆ET的含量增多 ;且推拉动作暴露所致损伤程度更重

EFFECT OF PUSH-PULL MANEUVER ON NO, NOS OF BRAIN TISSUE AND PLASMA ENDOTHELIN(ET) CONTENT OF RATS

To investigate the effect of push pull maneuver on nitric oxide (NO) and nitric oxide synthase (NOS)of brain tissue and plasma endothelin (ET), and to explore the mechanism of G induced loss of consciousness. Rats were given Gz and push pull maneuver, and contents of NO and NOS of the brain tissue and plasma ET were assayed 30 minutes, 3 hours,12 hours, 24 hours and 48 hours after the maneuver. The results showed that the contents of NO, NOS and ET of Gz group and push pull group were increased significantly at the time points of 30 minute, 3 hour and 12 hour as compared with those of the content group ( P <0 01). There was no statistical difference between the groups at 24h and 48h ( P >0 05). The contents of NO,NOS and plasma ET of push pull group showed statistically significant difference versus Gz group at 30 minutes, 3 hours and 12 hours ( P <0 05). It is concluded that high Gz and push pull maneuver may lead to an increase in the NO and ET in the hrain tissue and plasma ET content, and pash pull maneaver may induce heavier injury.