小鼠高脂血症造模方法的比较及优化

目的：通过不同造模方法的比较来建立新的高脂血症造模方法。方法：对四组小鼠分别进行正常喂养、乳剂灌胃、高脂饲料喂养和在高脂饲料喂养的同时隔天乳剂灌胃。然后在第0，7，14，21天检测小鼠血压以及血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）含量，并在实验结束时测定脏器系数。结果：乳剂＋高脂组肝系数显著高于其他组；血压值在14d后显著升高（P〈0．01）；TC含量和TG含量分别在7d和14d后显著升高（P〈0．01）；HDL-C含量在7d后显著性降低（P〈0．01）；第21天与第14天相比，血压和血脂水平能够继续维持，并且乳剂＋高脂组较乳剂灌胃组小鼠死亡率低。结论：本造模方法切实可行，在短期内效果明显。

Comparison and Optimization of the Method for Establishing the Hyperlipidemia Model in Mice

Objective： To develop a new method for establishment of hyperlipidemia animal model by comparing different methods. Method. Four groups of mice were given normal diet respectively,intragastric administration of fat emulsion once a day,high fat diet whole day and high fat diet whole day with given intragastric administration of fat emulsion every two days. The blood pressure and serum levels of TC, TG and HDL-C were measured at the first day, the seventh day,the fourteenth day and the twenty-first day. At the end of experimental period, the organ coeffcient was calculated. Result： Compared with other groups,the liver coefficient of the group with high fat diet whole day and intragastric administration of fat emulsion was statistically increased, the blood pressure level of this group were statistically increased after treatment of fourteen days （ P 〈 0. 01 ） ; The content of TC and TG statistically were increased after treatment of seven days and fourteen days, respectively （P 〈 0. 01 ） ;The content of HDL-C was decreased after treatment of seven days （P 〈0. 01 ） ;By comparison with the fourteenth day treatment,the blood pressure level and the high blood lipids level of the twenty-first day treatment stay steadily. The group with high fat diet whole day and intragastric administration of fat emulsion had low mice morality. Conclusion： The new method is practically workable and has a significant effect in a short time.