| 半边旗提取物5F对SPCA-1细胞周期及DNA、RNA合成的影响 |
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| 引用本文： | 戴滨,崔燎,吴铁,邓亦峰,吴科锋. 半边旗提取物5F对SPCA-1细胞周期及DNA、RNA合成的影响[J]. 中国临床药理学与治疗学, 2006, 11(4): 402-405 |
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| 作者姓名： | 戴滨 崔燎 吴铁 邓亦峰 吴科锋 |
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| 作者单位： | 1. 广东医学院药理教研室,湛江,524023,广东
2. 广东天然药物研究与开发实验室,湛江,524023,广东 |
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| 基金项目： | 国家自然科学基金;广东省科技厅科技攻关项目 |
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| 摘 要： | 目的：观察半边旗提取物5F对SPCA-1细胞的抑瘤作用及对细胞周期、DNA、RNA合成的影响.方法：用MTT法观察5F对SPCA-1细胞的抑瘤作用;流式细胞术分析细胞周期变化;[^3H]-TdR和[^3H]-UTR掺入法观察5F对SPC-A-1细胞DNA、RNA合成的影响.结果：5F对SPCA-1细胞有显著抑制生长作用;在一定剂量5F作用下SPCA-1细胞表现为G1期明显减少,而G2和S期相对增多;5F作用 24 h 后细胞[^3H]-TdR和[^3H]-UTR掺入量明显减少.结论：半边旗提取物5F具有体外抗肿瘤活性;干扰肿瘤细胞的周期;对肿瘤细胞DNA、RNA合成有抑制作用.
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| 关 键 词： | 半边旗 二萜类化合物 细胞周期 DNA合成 |
| 文章编号： | 1009-2501(2006)04-0402-04 |
| 收稿时间： | 2006-02-10 |
| 修稿时间： | 2006-03-22 |
Effect of compoud 5F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA and RNA of SPCA-1 cells |
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| DAI Bin,CUI Liao,WU Tie,DENG Yi-feng,WU Ke-feng. Effect of compoud 5F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA and RNA of SPCA-1 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(4): 402-405 |
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| Authors: | DAI Bin CUI Liao WU Tie DENG Yi-feng WU Ke-feng |
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| Affiliation: | Department of Pharmacology, Guang dong Medical College, Zhanjiang 524023, Guangdong, China;Guangdong Key Laboratory for Research and Development of Nature Drugs, Guang dong Medical College, Zhanjiang 524023, Guangdong , China |
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| Abstract: | AIM: To study the effects of compound 5F,one of the diterpenoids extracted from Pteris Semipinnata L,on cell cycle and synthesis of DNA and RNA of SPC-A-1 cells.METHODS: Cytotoxicity was determined by MTT test,the cell cycle was analysed by flowcytometry(FCM) and the synthesis of DNA and RNA was observed by the -TdR and -UTP incorporation.RESULTS: Compound 5F strongly inhibited the growth of SPCA-1cells,FCM analysis revealed that 5F could increase the percentage of cells in G_2 and S phase and decrease those in G_1 phase after 24 h treatment.The assays of -TdR and -UTP incorporation into DNA and RNA respectively showed obvious inhibition dose-dependent after exposure to 5F for 24 h.CONCLUSION: Compound 5F obviously inhibits SPC-A-1cells proliferation,arrests SPC-A-1cells in G2 and S phase and inhibits the synthesis of DNA and RNA. |
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| Keywords: | SPCA-1 |
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