| 聚合酶链反应法诊断结核病临床价值探讨 |
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| 引用本文： | 刘辉国,熊盛道,熊薇,王斌.聚合酶链反应法诊断结核病临床价值探讨[J].临床内科杂志,1999,16(5):247-249. |
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| 作者姓名： | 刘辉国 熊盛道 熊薇 王斌 |
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| 作者单位： | 430030,武汉,同济医科大学附属同济医院呼吸科 |
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| 摘 要： | 目的 探讨ＰＣＲ法对结核病的诊断价值。方法 用ＰＣＲ法检测痰、纤支镜抽吸物、胸水和血液标本中结核杆菌ＤＮＡ。结果 ＰＣＲ法敏感性为２１．９％，特异性为９２．６％，总的阳性预测值、阴性预测值和诊断效率分别为６４．６％，６５．９％和６５．８％，不同标本敏感性有较大的差异。结论 ＰＣＲ法诊断结核病的敏感性、特异性尚远不能完全满足临床的需要，需进一步完善和改进 。
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| 关 键 词： | 结核/诊断 聚合酶链反应 |
Study on diagnostic value of PCR in tuberculosis |
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| Liu Huiguo,Xiong Shengdao,Xiong Wei,et al..Study on diagnostic value of PCR in tuberculosis[J].Journal of Clinical Internal Medicine,1999,16(5):247-249. |
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| Authors: | Liu Huiguo Xiong Shengdao Xiong Wei |
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| Institution: | Liu Huiguo,Xiong Shengdao,Xiong Wei,et al.Tongji Hospital,Tongji Medical University,Wuhan 430030 |
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| Abstract: | Objective To study the diagnostic value of PCR in tuberculosis.Methods To detect mycobacterium tuberculosis DNA by PCR tecnique in 901 specimens,including sputum,bronchial lavage fluid,pleural effusion and blood.Results The sensitivity and specificity of PCR was 21.9% and 92.6%,respectively.Total positive predictive value,negative predictive value and diagnosis efficiency was 64.6%,65.9%and65.8%,respectively.Conclusion PCR techinque may be useful on diagnosis of tuberculosis,but at present the sensitivity and specificity are too low to meet clinical need. |
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| Keywords: | Tuberculosis/ diagnosis Polymerase Chain Reaction |
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