结核性脑膜炎患者脑脊液S100B与一氧化氮合酶的相关性研究

目的:探讨结核性脑膜炎患者脑脊液S100B、一氧化氮合酶(iNOS)含量的动态变化及两者的相关性。方法:43例结核性脑膜炎患者(TM组)和无神经系统疾病的患者28例(对照组)均于发病后1-6 d首次采集脑脊液,以后每隔5-7 d采集1次,共5次。采用酶联免疫吸附试验(ELISA)、化学比色法分别测定2组患者不同病程脑脊液S100Bi、NOS含量。结果:发生昏迷(昏迷组)15例,未发生昏迷(无昏迷组)28例。昏迷组和无昏迷组不同病程脑脊液S100Bi、NOS含量均较对照组显著增高(P<0.05);昏迷组脑脊液S100Bi、NOS含量较无昏迷组明显增高(P<0.05);昏迷组和无昏迷组患者脑脊液S100B与iNOS含量均呈高度相关(P=0.0319,0.0337)。结论:脑脊液S100B作为脑损害的标志物,与iNOS高度相关,其含量的高低能够反映结核性脑膜炎患者病情的严重程度。

Clinical Significance of S100B and iNOS in Cerebrospinal Fluid from Patients with Tuberculous Meningitis

Objective:The relation between the dynamical change of S100B and iNOS in cerebrospinal fluid(CSF) in patients with tuberculous meningitis(TM) has been investigated.Methods: 43 patients with TM(TM group) and 28 patients without never system diseases(control group) have been studied.S100B was measured by ELISA and iNOS by chemistry colorimetry.Results: Both S100B and iNOS levels in CSF from the patients with TM were significantly increased in contrast to those in the controls(P<0.05). S100B and iNOS levels in the comatose patients were significantly higher than those in the conscious ones(P<0.05).Further,S100 and iNOS levels have close correlation in the comatoze patients and the conscious patients(P=0.0319,0.0337). Conclusion: S100B in CSF as a marker of brain injury was closely associated with iNOS,and levels could reflex the degree of brain damage in patients with TM.