| 过表达PRDM13抑制胶质瘤细胞相关功能基因的测序分析 |
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| 引用本文： | 张琳娜,何涛,宫伟,陶虹,曹慧梅,杨吉娟,扈启宽.过表达PRDM13抑制胶质瘤细胞相关功能基因的测序分析[J].临床医学工程,2019(2):159-161. |
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| 作者姓名： | 张琳娜 何涛 宫伟 陶虹 曹慧梅 杨吉娟 扈启宽 |
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| 作者单位： | 1.宁夏医科大学基础医学院生理学系;2.湖北医药学院附属国药东风总医院脊柱外科;3.宁夏人民医院骨科;4.宁夏颅脑疾病重点实验室/国家重点实验室培育基地 |
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| 基金项目： | 宁夏自然科学基金项目(项目编号:NZ17085) |
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| 摘 要： | 目的比较过表达PRDM13慢病毒与感染阴性对照病毒的胶质瘤细胞mRNA存在的差异,筛选并分析抑制胶质瘤增殖、迁移相关的基因。方法通过胶质瘤细胞感染慢病毒,建立过表达PRDM13细胞模型,使用转录组测序方法检测两组中差异表达基因,并进行GO分析。结果过表达PRDM13慢病毒细胞模型成功建立,过表达PRDM13可以改变U87细胞的形态,转录组测序检测到实验组和对照组显著性差异表达基因。结论找到抑制胶质瘤增殖、迁移的相关基因,为进一步研究PRDM13的作用机制提供了理论依据。
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| 关 键 词： | 胶质瘤细胞 PRDM13 转录组测序 |
Sequencing Analysis of Inhibition Glioma Cell Related Functional Genes by Overexpression PRDM13 |
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| ZHANG Linna,HE Tao,GONG Wei,TAO Hong,CAO Huimei,YANG Jijuan,HU Qikuan.Sequencing Analysis of Inhibition Glioma Cell Related Functional Genes by Overexpression PRDM13[J].Medical and Health Care Instruments,2019(2):159-161. |
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| Authors: | ZHANG Linna HE Tao GONG Wei TAO Hong CAO Huimei YANG Jijuan HU Qikuan |
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| Institution: | (Department of Physiology of Basic Medical College,Ningxia Medical University,Yinchuan 750004,China;Department of SpinalSurgery,Dongfeng General Hospital of Chinese Medicine Affiliated to Hubei Medical University,Shiyan 442000,China;Departmentof Orthopedics,Ningxia People's Hospital,Yinchuan 750004,China;Ningxia Key Lab of Cerebrocranial Diseases / The National KeyLaboratory Incubation Base,Yinchuan 750004,China) |
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| Abstract: | ZHANG Linna;HE Tao;GONG Wei;TAO Hong;CAO Huimei;YANG Jijuan;HU Qikuan(Department of Physiology of Basic Medical College,Ningxia Medical University,Yinchuan 750004,China;Department of SpinalSurgery,Dongfeng General Hospital of Chinese Medicine Affiliated to Hubei Medical University,Shiyan 442000,China;Departmentof Orthopedics,Ningxia People's Hospital,Yinchuan 750004,China;Ningxia Key Lab of Cerebrocranial Diseases / The National KeyLaboratory Incubation Base,Yinchuan 750004,China) |
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