Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples |
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| Authors: | Joel Isanga Bitange Nipa Tochi Daniel Mukunzi Yanni Chen Liqiang Liu Hua Kuang |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People’s Republic of China;2. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People’s Republic of China;3. Department of Biochemistry, College of Natural Sciences, Makerere University, Kampala, Uganda;4. Department of Food Science and Technology, Pwani University, Kilifi, Kenya |
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| Abstract: | ![]()
A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC50) of MAb was 0.41?ng/ml with a limit of detection (LOD) of 0.15?ng/ml. The ELISA results were obtained within 90?min. The mean recoveries from all the spiked food samples were within the range of 79.02–105.49%, with coefficients of variation in the range of 2.21–11.4%. The strip test results obtained within 5?min had visual LODs in the range 2.5–5?µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods. |
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| Keywords: | Apramycin monoclonal antibody indirect competitive enzyme-linked immunosorbent assay rapid strip test |
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