化疗对卵巢癌患者免疫功能的影响

目的；研究化疗对卵巢癌患者细胞免疫功能的影响及其临床意义。方法：应用间接免疫荧光法、MTT比色法、ELISA检测围手术期卵巢癌患者外周血淋巴细胞（PBL）T淋巴细胞亚群、自然杀伤（NK）活性及血清可溶性白介素2受体（rIL-2R)，结果：卵巢癌患者PBL中CD3^ ,CD4^ ,CD4^ /CD8^ 均明显低于良性肿瘤组，而ac^ ，明显升高，化疗后患者CD3^ ,CD4^ 明显降低，而rIL-2R,NK活性无明显改变。结论；化疗可使卵巢癌患者细胞免疫功能明显受抑，且恢复困难，需选择适当免疫治疗以提高机体自身抗瘤活性。

Effects of chemotherapy on the immune status in patients with malignant ovarian tumor

Objective To evaluate the effect of cisplatin-based chemotherapy on the immunity of patient with malignant ovarian tumor (MOT) . Methods T-lymphocyte subsets, natural killer (NK) activity and the levels of soluble interleukin-2 receptors (rIL-2R) were measured in 25 patients with MOT and 26 benign controls (BC) by sandwich ELISA, indirect immunofluorescence and MTT colorimetry. Results The numbers of CD3+ , CD4+ , and CD4+/CD8+ in the patients were lower than those in the control significantly, but Tac+ increased markedly (P< 0.05) . The CD3+ , CD4+ decreased remarkbly after chemotherapy, while the NK activity and rIL-2R with no significant changes. Conclusion The immune status of MOT is depressed during the course of chemotherapy, and is very difficult to improve it. Therfore suitable immunotherapy is needed.