| 不同提取方法测定野马追药材中绿原酸含量比较研究 |
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| 引用本文： | 崔伟伟,范永春.不同提取方法测定野马追药材中绿原酸含量比较研究[J].辽宁中医学院学报,2011(3):178-180. |
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| 作者姓名： | 崔伟伟 范永春 |
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| 作者单位： | 江苏省中西医结合医院,江苏南京210028 |
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| 摘 要： | 目的：研究野马追药材中绿原酸含量测定的前提取方法及绿原酸含量的质控标准。方法：采用高效液相色谱法,比较了不同的提取方法、提取溶媒、提取时间和溶媒用量测定野马追药材中绿原酸含量。结果：采用超声提取方法提取野马追药材中绿原酸,且绿原酸高效液相法测定进样量在0.0908～1.362μg（r=0.9999）间呈良好的线性关系,平均回收率为100.12%,RSD为1.31%。结论：绿原酸超声提取方法和高效液相测定含量方法简便,可用于该药材的质量控制。
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| 关 键 词： | 野马追 绿原酸 不同提取方法 高效液相色谱 含量测定 |
Comparative Study of Different Extraction Methods to Determine Chlorogenic Acid Content in YMZ |
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| CUI Wei-wei,FAN Yong-chun.Comparative Study of Different Extraction Methods to Determine Chlorogenic Acid Content in YMZ[J].Journal of Liaoning College of Traditional Chinese Medicine,2011(3):178-180. |
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| Authors: | CUI Wei-wei FAN Yong-chun |
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| Institution: | (Jiangsu Provincial Hospital of Intergrated Traditional Chinese and Western Medicine,Nanjing 210028,Jiangsu,China) |
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| Abstract: | Objective：To study the YMZ in the content determination of chlorogenic acid in the extraction methods and quality control of chlorogenic acid standards.Methods：High performance liquid chromatography to compare the different extraction methods,extraction solvent,extraction time and solvent consumption wild chase determination of chlorogenic acid content of herbs.Results：The use of ultrasonic extraction method to recover the herbs in chlorogenic acid,and chlorogenic acid by HPLC with sampling in the 0.09081.362μg（r = 0.9999）showed a good linear relationship between the average recovery was 100.12%,RSD 1.31%.Conclusion：The ultrasonic extraction of chlorogenic acid content was determined by high performance liquid method is simple and can be used for quality control of the medicine. |
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| Keywords: | Y-MZ chlorogenic acid different extraction methods HPLC content determination |
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