| HPLC法测定清痤颗粒中绿原酸的含量 |
| |
| 引用本文： | 许永,吕晓燕. HPLC法测定清痤颗粒中绿原酸的含量[J]. 安徽医药, 2012, 16(3): 316-317 |
| |
| 作者姓名： | 许永 吕晓燕 |
| |
| 作者单位： | 安徽省六安市中医院,安徽六安,237006 |
| |
| 摘 要： | 目的建立用高效液相色谱法测定清痤颗粒中绿原酸含量的方法。方法采用HPLC法,依利特C18色谱柱(250 mm×4.6 mm,5μm),乙腈:0.4%磷酸溶液13∶87为流动相,检测波长327 nm,柱温30℃,流速1.0 ml.min-1。结果绿原酸浓度范围在10.08～60.48 mg.L-1之间峰面积(Y)与浓度(X)线性关系良好。平均回收率95.08%,RSD=2.13%。结论该方法操作简单,分离效果好,可用于清痤颗粒制剂质量控制。
|
| 关 键 词： | 清痤颗粒 绿原酸 高效液相色谱法 |
Determination of chlorogenic acid in qingcuo granule by HPLC |
| |
| XU Yong , L Xiao-yan. Determination of chlorogenic acid in qingcuo granule by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2012, 16(3): 316-317 |
| |
| Authors: | XU Yong L Xiao-yan |
| |
| Affiliation: | XU Yong,Lü Xiao-yan(Luan Traditional Chinese Medicine Hospital,Luan 237006,China) |
| |
| Abstract: | Objective To establish a method for the determination of chlorogenic acid in qingcuo granule by HPLC.Methods An HPLC method was established.The chromatographic column was Elite C18（250 mm ×4.6 mm,5 μm）.The mobile phase was acid-acetonitrile：0.4% phosphoric（13∶ 87） with the flow rate of 1.0 ml·min-1,and the detection wavelength was 327 nm.The column temperature was 30℃.Results The linear range of chlorogenic acid was from 10.08 mg·L-1 to 60.48 mg·L-1.The average recovery was 95.08%,and RSD was 2.13%.Conclusion The method is simple and has good separation efficiency,which can be used for quality control of qingcuo granule. |
| |
| Keywords: | qingcuo granule chlorogenic acid HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
