GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells |
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Authors: | Geng Degui Joshi Sunil K Podolsky Robert She Jin-Xiong |
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Affiliation: | Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Room CA4124, 1120 15th Street, Augusta, GA 30912, United States. |
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Abstract: | RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lalpha, IL-lbeta, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFbeta, TNFalpha and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells. |
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