三七总皂苷对兔BMSCs的成骨分化及TGF-β1表达的影响

目的:观察三七总皂苷(panax notoginseng saponins,PNS)对兔骨髓间充质干细胞(bone marrow stromal cells,BMSCs)成骨分化及TGF-β1在基因水平表达变化的影响。方法:体外分离培养兔BMSCs,观察不同浓度PNS(50、100、200 mg/L)对兔BMSCs的影响,采用MTT法检测细胞增殖,茜素红染色观察钙结节形成,qRT-PCR检测TGF-β1表达水平。结果:各浓度PNS培养下的细胞增殖无明显差异(P>0.05);100、200 mg/L PNS组的钙结节数量和TGF-β1的表达水平均高于阴性组(P<0.05)。结论:PNS可促进兔BMSCs的成骨分化并刺激其分泌TGF-β1,但无明显促细胞增殖作用。

Panax Notoginseng Saponins Mediate Rabbit Bone Marrow Mesenchymal Stem Cells Osteodifferentiation

Objective： To observe the osteoinduction effect of panax notoginseng saponins （PNS） on rabbit bone marrow mesenchymal stem cells （BMMSCs）, and the expression level change of TGF-β1. Methods： Rabbit BMMSCs were isolated and cultured in vitro. To observe the influence of different concentration of PNS （50、100、200 mg/L） on rabbit BMMSCs,several methods were performed, including cell culture technique, MTT colorimetric assay, Dahl McGec Russell＇s Alizarin red staining for calcium and qRT-PCR explore TGF-β1 expression level. Results： There was no significant difference of proliferation capacity of BMMSCs between various concentration of PNS （P 〉0.05）. The calcium nodule and TGF-β1expression level in 100、200 mg/L PNS groups were significantly higher than negative group （P〈0.05）. Conclusion： PNS can promote the osteogenic differentiation and secretion of TGF-β1 of rabbit BMMSCs, but PNS is not effective enough for mediation of BMMSCs proliferation.