食管鳞状细胞癌患者microRNA-127-3p 的 表达水平及临床意义

目的 探讨miR-127-3p 在食管鳞状细胞癌（ESCC）中的表达及其临床意义。方法 选取 2013 年2 月—2015 年2 月于湖南省人民医院和长沙市中心医院行外科手术的80 例ESCC 患者癌组织及癌 旁组织标本，采用实时荧光定量聚合酶链反应检测miR-127-3p mRNA 相对表达量，利用免疫组织化学法 检测SKI 蛋白在患者癌组织及癌旁组织标本中的表达，并对所有患者进行≥ 3 年的随访。同时检测ESCC 细 胞株Eca109 和Kyse150 及正常人食管上皮细胞株Het-1a 中miR-127-3p 的表达水平。结果 免疫组织化 学染色结果表明，癌组织中SKI 蛋白表达阳性率较癌旁组织升高（P <0.05）；ESCC 细胞株Eca109、Kyse150 中miR-127-3p 相对表达量低于正常人食管上皮细胞株Het-1a（P <0.05）；临床分期Ⅲ期患者miR-127- 3p 低表达率高于Ⅰ期和Ⅱ期（P <0.05），有淋巴结转移和饮酒史患者miR-127-3p 低表达率高于无淋巴 结转移和无饮酒史患者（P <0.05），miR-127-3p 低表达患者的中位无进展期较高表达患者短（P <0.05）。 结论 miR-127-3p 通过靶向调控SKI 蛋白的表达参与ESCC 的发生，其低表达可能参与ESCC 的侵袭及转 移过程，并显著影响患者的预后。

Expression and clinical significance of miR-127-3p in patients with esophageal squamous cell carcinoma

Objective To investigate the expression and clinical significance of miR-127-3p in esophageal squamous cell carcinoma (ESCC). Methods Cancer and adjacent tissues of eighty patients with ESCC from February 2013 to February 2015 were enrolled in this study. The expression of miR-127-3p was detected by real-time PCR (Polymerase Chain Reaction), the expression of SKI (V-Ski Sarcoma Viral Oncogene Homolog) in cancer tissues and adjacent tissues was detected by immunohistochemistry, and all cases were followed up for at least 3 years. The expression levels of miR-127-3p in ESCC cell lines Eca109 and Kyse150 and normal human esophageal epithelial cell line Het-1a were also detected. Results The relative expression of miR-127-3p in ESCC patients was lower than that in adjacent tissues (P < 0.05). The relative expression of miR-127-3p in ESCC cell lines Eca109 and Kyse150 was lower than that in normal human esophageal epithelial cell lines Het-1a, and the difference was statistically significant (P < 0.05). The low expression rate of microRNA-127-3p in patients with clinical stage III was higher than that in patients with clinical stage I and II (P < 0.05); that in patients with lymph node metastasis and drinking history was higher than that in patients without lymph node metastasis and drinking history (P < 0.05); the median progression-free period of patients with low expression of microRNA-127-3p was shorter than that in patients without lymph node metastasis and drinking history (P < 0.05). Conclusions miR-127-3p participates in the development of ESCC by regulating expression of SKI protein, and its low expression may be involved in the invasion and metastasis of ESCC, and significantly affect the prognosis of patients.