IL-2活化瘤苗对胃癌术后复发和转移的抑制作用

目的观察用细胞因子活化的特异性瘤苗辅助常规化疗,治疗外科手术后临床Ⅲ～Ⅳ期胃癌患者,抑制肿瘤术后复发、转移的临床疗效。方法于外科术后常规化疗结束后2周,用患者自体肿瘤细胞抗原致敏、IL-2活化的树突状细胞(DC)作为瘤苗给患者四肢皮下多点免疫注射1×106/(ml·次),每周1次,每疗程注射4次,0.5年后强化1次。定期胃镜、影象学和肿瘤标志物检查,随访至手术后第2年,观察生存期和生存率。结果经瘤苗治疗后,患者的免疫功能显著改善。术后24个月,瘤苗联合化疗组患者的生存率为50%,血清中CEA和CA724分别为(10±1.5)ng/ml和(5.5±1.5)U/ml,而单纯化疗组患者的生存率为10%,CEA和CA724的水平分别为(77.0±9.4)ng/ml和(55.0±7.6)U/ml;P<0.001。结论特异性瘤苗免疫治疗,能增强胃癌患者术后的特异性抗肿瘤免疫功能,提高手术后化疗疗效,抑制肿瘤复发和转移,能延长患者生存期,提高生存率。

Clinical studies on inhibiting effects of specific auto-tumor vaccines activated by IL-2 on postoperative tumor recurrence and metastasis of gastric carcinoma

Objective To observe the treatment effect of specific vaccines of auto-tumor cell antigen pulsed dendritic cells(DC)activated by interleukin2(IL-2)on postoperative tumor recurrence and metastasis of advanced gastric carcinoma(GC).Method Auto-tumor cell antigen separated from fresh tumor tissue of GC patients was prepared.Before and after immunotherapy,the fluorescence of costimulatory molecules on peripheral blood mononuclear cells(Mo)of the patients was analyzed by FACS.The vaccines were multi-injected with1×106/(ml·per capita)to patients,subcutaneous once a week.Four times was a course,and enhanced once later six months after treatment.Observing treatment effect was taken later twenty-four months after surgery.Results After immunotherapy,the immune function of the patients was enhanced significantly.Twenty-four months after surgery,the survival rate was50%,the levels of CEA and CA724in the immunotherapy group was(10±1.5)ng/ml and(5.5±1.5)U/ml,respectively;the chemo-therapy group of chemotherapy was10%,the levels of CEA and CA724was(50.5±13.0)ng/ml and(22.0±2.7)U/ml,respectively,P<0.001.Conclusion Immunotherapy with the vaccine can enhance specific anti-tumor immune function and effect of chemotherapy in GC,and inhibits postoperative tumor recurrence and metastasis.