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| 对血液抗-HCV筛查结果的确认分析 | |
| 引用本文： | 张健,黄守民,李雪丽,黄建国,邓爱玲. 对血液抗-HCV筛查结果的确认分析[J]. 现代检验医学杂志, 2002, 17(1): 31-32 |
| 作者姓名： | 张健 黄守民 李雪丽 黄建国 邓爱玲 |
| 作者单位： | 深圳市宝安区血站,广东深圳,518101 |
| 摘 要： | 目的 探讨对抗－HCV ELISA阳性血液进行确认分析的重要性。方法 用第三代免疫印迹法（RIBA 3．0）对抗HCV ELISA阳性血液进行确认，并对部分样品用荧光定量聚合酶链反应（FQ－PCR）检测HCV－RNA。结果 80份抗HCV ELISA阳性血样经RIBA确认，其中阳性38份（47．5％），可疑20份（25％），阴性22份（27．5％），c22、c33c的检出率高于c-100和NS5，而且20份RIBA 3．0可疑血样的HCV－RNA为阴性。结论 对抗－HCV阳性血液确认尤为重要，能提高诊断HCV的准确性。 |
| 关 键 词： | 丙型肝炎病毒 免疫印迹法 诊断 荧光定量聚合酶链反应 |
| 文章编号： | 1005-1716(2002)01-031-02 |
| 修稿时间： | 2001-04-20 |
Evaluation on Confirmatory Test for Detecting Antibody against HCV |
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| ZHANG Jian,HUANG Shou-min,LI Xue-li,HUANG Jian-guo,DENG Ai-ling. Evaluation on Confirmatory Test for Detecting Antibody against HCV[J]. Journal of Modern Laboratory Medicine, 2002, 17(1): 31-32 | |
| Authors: | ZHANG Jian HUANG Shou-min LI Xue-li HUANG Jian-guo DENG Ai-ling |
| Abstract: | Objective To evaluate the importance of confirmatory test for detecting antibody against HCV ELISA(+).Methods Anti-HCV ELISA(+) samples were confirmed by RIBA 3.0,and some of them were detected by FQ-PCR.Results 80 anti-HCV ELISA(+) samples were confirmed by RIBA 3.0,38(47.5%)were positive,20(25%) were indeterminate but HCV-RNA of them were negative,and 22(27.5%) were negative.Also the positive rate of c22 and c33c was higher than that of c-100 and NS5.Conclusion The results show that anti-HCV ELISA(+) samples should be confirmed by RIBA 3.0 in order to ensure the accuracy of diagnose. |
| Keywords: | HCV RIBA diagnose:FQ-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |