酒速愈对急性酒精中毒小鼠海马AchE和纹状体单胺神经递质含量的影响

目的探讨酒速愈对急性酒精中毒小鼠脑海马胆碱酯酶(AchE)活性和纹状体多巴胺(DA)、5羟色胺(5-HT)含量的影响。方法采用56度红星二锅头酒灌胃建立急性酒精中毒小鼠模型,以葛花解酲汤为对照,观察酒速愈对急性酒精中毒小鼠醉酒时间、醉酒率以及脑海马AchE活性和纹状体DA和5-HT含量的影响。结果模型组小鼠出现明显先兴奋后抑制的表现,脑海马AchE活性和纹状体DA和5-HT的含量较正常组升高(P<0.01),与模型组比较,酒速愈高、低剂量组可明显降低小鼠醉酒率(P<0.05),延长醉酒时间(P<0.05或P<0.01);且酒速愈高剂量组醉酒时间长于葛花解酲汤组(P<0.05),同时3治疗组均可明显降低纹状体DA和5-HT的含量(P<0.05或P<0.01),酒速愈高剂量组和葛花解酲汤组可明显降低脑海马AchE活性(P<0.05或P<0.01)。结论酒速愈可调节脑组织单胺类神经递质DA、5-HT含量以及AchE的活性,改善急性酒精中毒时神经行为异常。

Influence of Jiusuyu Suspension on AchE activity of hippocampus and striatum monamine neurotransmitter content in acute alcoholism mice

Objective To investigate the influence of Jiusuyu Suspension on hippocampus acetylcholinesterase (AchE) activity and striatum dopamine (DA) and 5-hydroxytryptamine (5-HT) contentsin acute alcoholism mice.Method The mouse model of acute alcoholism was established by giving Hongxing Erguotou Spirit (containing 56 per cent alcohol) to mice orally. The influence of Jiusuyu Suspension on drunken time, drunken rate, hippocampus AchE activity and striatum contents of DA and 5-HT were observed and compared with Gehuajiecheng Decoction.Result The rats of the model group showed an exciting at the first and repressive manifestations then. The activity of hippocampus AchE and striatum contents of DA and 5-HT in the model group were increased compared with those in the normal group (P<0.01). The drunken rate was obviously decreased (P<0.05) and drunken time was prolonged (P<0.05 or P<0.01) in the groups with high or low dose of Jiusuyu Suspension compared with those in the model group. The drunken time was longer in the group with high dose of Jiusuyu Suspension than that in Gehuajiecheng Decoction group (P<0.05). The contents of DA and 5-HT were obviously reduced in 3 treatment groups (P<0.05 or P<0.01). The activity of hippocampus AchE was obviously decreased in the group with high dose of Jiusuyu Suspension and Gehuajiecheng Decoction group (P<0.05 or P<0.01).Conclusion Jiusuyu Suspension can regulate the contents of brain monoaminergic neurotransmitter (DA, 5-HT) and AchE activity, and relieve the abnormal neurobehaviors of alcoholism.