2015年我国17省市5岁以下腹泻患儿星状病毒感染监测结果分析

目的 了解2015年我国17省市5岁以下腹泻患儿星状病毒的流行特征.方法 收集2015年我国17省市4 672份5岁以下腹泻患儿的腹泻粪便样本和相应病例临床信息,采用描述性流行病学方法分析星状病毒感染的分布特征.利用逆转录-聚合酶链反应(RT-PCR)反应进行星状病毒检测,对其中部分星状阳性PCR产物测序并进行系统进化分析.结果 星状病毒检出的阳性率约为3.40％(159/4 672).星状病毒感染阳性的患儿年龄主要集中在0～2岁,占85.53％(136/159);季节高峰在1月;流行地区主要位于西部及北部地区.选取52份星状病毒阳性标本进行测序,获得32株序列,系统进化分析结果显示,31株为经典HAstV-1型星状病毒,同时检测到1株HAstV-5型星状病毒.结论 星状病毒是我国17省市5岁以下腹泻患儿的重要病原体之一,经典的HAstV-1型是我国星状病毒流行优势株.

Surveillance of astrovirus infection in children under 5 years old with diarrhea in seventeen provinces of China, 2015

Objective To investigate the molecular epidemiological characteristics of astrovirus in children with diarrhea under 5 years old from seventeen provinces of China,2015.Methods A total of 4672 specimens from gastroenteritis patient under 5 years old were collected with relevant clinical data.The astroviruses in specimens were detected by RT-PCR.Some of the astrovirus positive PCR products were sequenced and analyzed for phylogenetics.Results The total positive rate of astrovirus was 3.40％ (159/4 672).The astrovirus positive rate was the highest in age group 0-2 accounting for 85.53％ (136/159).The seasonal peak occurred in January.The high detection rate of astrovims occurred in Western and Northern area of China.Of the 52 astrovirus positive strains which were selected,32 strains were sequenced successfully.Thirty-one strains were classical Hasty-1 astrovirus while one strain was classical Hasty-5 astrovirus.Conclusions Astrovirus is one of the important diarrheal pathogens in children under five years old in China.Classic Hasty-1 astrovirus was the predominant strain.