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| 介绍一种大量纯化钙调素的方法 | |
| 引用本文： | 钱迅 文允镒. 介绍一种大量纯化钙调素的方法[J]. 基础医学与临床, 1996, 16(6): 468-472 |
| 作者姓名： | 钱迅 文允镒 |
| 作者单位： | 中国医学科学院基础医学研究所生理研究室 |
| 摘 要： | 本文介绍了一种适于大量制备钙调素的简易实用方法。，我们用该方法从２．５ｋｇ猪脑组织中一次纯化了１５０ｍｇ，经鉴定，所提ＣａＭ的纯度在ＳＤＳ ＰＡＧＥ中呈一区带，分子量为１９ｋＤ，等电点为４．３８。并对ＰＤＥ有明显的激活作用，该作用可被特异性抑制剂ＴＦＰ抑制。 |
| 关 键 词： | 钙调素 分离纯化 |
A Large Scale Purification Method of Calmodulin |
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| Qian Xun, Wen Yunyi, Chen Mengqin, Wang Shuxia. A Large Scale Purification Method of Calmodulin[J]. Basic Medical Sciences and Clinics, 1996, 16(6): 468-472 | |
| Authors: | Qian Xun Wen Yunyi Chen Mengqin Wang Shuxia |
| Abstract: | simple and a large amount purification method of calmtalulin(CaM)from porcine brain wasperformed by modified hydrophobic affinity chromatography. With this method,150mg CaM from 2.5kg porcine brain was prepared.The purity,molecu-lar weight,isoelectric point,and activating effect of the product on PDE were studied.This method has following advantages: The whole precedure was more simple and efficientthat 60 mg CaM could be obtained from 500g porcine brain,By this method,the amount of caMwas yielded more higher than that of others. |
| Keywords: | calmodulin isolation and purification hydrophobic affinity chromatography ion exchange chromatography |
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