藏药马尿泡离体快繁技术研究

目的 通过对马尿泡组织培养技术的研究，为马尿泡的快速繁殖提供技术支撑。方法 以马尿泡野生植株上的休眠芽为外植体，将它们接种到一系列含有不同激素的培养基中。结果 外植体野生芽不易进入正常萌发生长阶段，一旦进入正常阶段，能迅速进入生长状态，诱导野生芽分化的最适培养基是MS+6-BA 2.0 mg/L+NAA 1.0 mg/L，且侧芽要比主芽分化得快，增殖率明显高于主芽的增殖率。25 d转接1 次，增殖倍数在5倍以上。最适生根培养基是1/2 MS+IBA 0.5 mg/L，诱导生根率达96%。无菌苗幼叶诱导愈伤组织最适培养基是MS+6-BA 0.5 mg/L+NAA 0.5 mg/L+2，4-D 1.0 mg/L，诱导率达100%。结论 一方面建立了稳定高效的马尿泡再生体系，另一方面通过组织培养达到了其种质资源的保存。

Rapid propagation in a Tibetan medicine Przewalskia tangutica

Objective To provide scientific basis for rapid propagation of Przewalskia tangutica by means of preliminary study of the tissue culture technique. Methods Using the dormant wild buds from P. tangutica as explants to inoculate the buds on a series of media, which contained various hormone concentration. Results It was difficult for the dormant wild bud to get into germinating and growing periods. Conversely, it would rapidly develop. MS + 6-BA 2.0 mg/L + NAA 1.0 mg/L was the optimum medium for inducing differentiation of the dormant wild bud, and the lateral bud was more appropriate for differentiation than the apical bud, and the rate of proliferation of the lateral bud was higher than that of the apical bud. The multiple of the proliferation was above five times if it was transferred every twenty five days. 1/2 MS + IBA 0.5 mg/L was the optimum medium for root induction, and the rate of the root induction was highly up to 96%. MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L + 2, 4-D 1.0 mg/L was the optimum medium for inducing callus using the young leaves of sterile seedling. Conclusion A stable potential regeneration system is established and the idioplansm resources would be preserved by means of the tissue culture technique.