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| 钙敏感受体通过PI3K/AKT通路对缺氧诱导的A549细胞增殖的影响 | |
| 引用本文： | 李光伟,苗宏志,李波,沈云虹,邱丽萍,张亚珍,金香兰,朱坤杰. 钙敏感受体通过PI3K/AKT通路对缺氧诱导的A549细胞增殖的影响[J]. 中国病理生理杂志, 2017, 33(3): 505-509. DOI: 10.3969/j.issn.1000-4718.2017.03.020 |
| 作者姓名： | 李光伟 苗宏志 李波 沈云虹 邱丽萍 张亚珍 金香兰 朱坤杰 |
| 作者单位： | 1. 齐齐哈尔医学院病理生理教研室, 黑龙江 齐齐哈尔 161006; 2. 齐齐哈尔市第一医院胸心外科, 黑龙江 齐齐哈尔 161006; 3. 齐齐哈尔医学院机能实验室, 黑龙江 齐齐哈尔 161006 |
| 基金项目： | 齐齐哈尔医学院博士基金资助项目（No.QY2016B-16） |
| 摘 要： | 目的:探讨钙敏感受体(calcium-sensing receptor,CaSR)在缺氧诱导的A549及A549/DDP细胞增殖中的信号转导途径。方法:通过缺氧培养箱内(93%N_2、2%O_2、5%CO_2)培养24 h的方法复制细胞缺氧模型。应用Western blot技术分析PCNA及p-AKT蛋白不同处理情况下的表达水平;采用流式细胞技术检测不同处理因素对细胞周期及增殖指数的影响,应用BrdU掺入法分析不同处理因素对细胞DNA合成的影响。结果:缺氧引起A549及A549/DDP细胞PCNA及p-AKT蛋白水平上调,增加BrdU掺入量、S期细胞数量和细胞增殖指数,GdCl_3能够增强缺氧的作用,但上述效应可以被LY294002抑制。结论:PI3K/AKT信号通路在缺氧活化CaSR并促进A549及A549/DDP细胞增殖中起重要作用。 |
| 关 键 词： | 钙敏感受体 A549细胞 缺氧 细胞增殖 PI3K/AKT信号通路 |
| 收稿时间： | 2016-10-09 |
Calcium-sensing receptor mediates hypoxia-induced proliferation of A549 cells through PI3K/AKT pathway |
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| LI Guang-wei,MIAO Hong-zhi,LI Bo,SHEN Yun-hong,QIU Li-ping,ZHANG Ya-zhen,JIN Xiang-lan,ZHU Kun-jie. Calcium-sensing receptor mediates hypoxia-induced proliferation of A549 cells through PI3K/AKT pathway[J]. Chinese Journal of Pathophysiology, 2017, 33(3): 505-509. DOI: 10.3969/j.issn.1000-4718.2017.03.020 | |
| Authors: | LI Guang-wei MIAO Hong-zhi LI Bo SHEN Yun-hong QIU Li-ping ZHANG Ya-zhen JIN Xiang-lan ZHU Kun-jie |
| Affiliation: | 1. Department of Pathophysiology, Qiqihar Medical University, Qiqihar Medical University, Qiqihar 161006, China; 2. Department of Thoracic and Cardiovascular Disease, The First Hospital of Qiqihar, Qiqihar Medical University, Qiqihar 161006, China; 3. Department of Functional Laboratory, Qiqihar Medical University, Qiqihar 161006, China |
| Abstract: | AIM: To explore the cell signal transduction pathway of calcium-sensing receptor (CaSR) mediating hypoxia-induced proliferation of A549 cells and A549/DDP cells. METHODS: The cell proliferation was tested by BrdU incorporation assay. The cell cycle analysis was carried out by flow cytometry. The protein levels of PCNA and p-AKT were analyzed by Western blot. RESULTS: Hypoxia significantly increased the protein levels of PCNA and p-AKT, the BrdU incorporation and the cell proliferation index. GdCl3 (an agonist of CaSR) amplified the effect of hypoxia. LY294002 (a PI3K inhibitor) decreased the up-regulation of PCNA expression, the BrdU incorporation and the increased cell proliferation index induced by hypoxia and GdCl3 in the A549 cells and A549/DDP cells. CONCLUSION: CaSR is expressed in A549 cells and 549/DDP cells, and CaSR activation induced by hypoxia promotes the proliferation of A549 cells and A549/DDP cells through PI3K/AKT signaling pathway. |
| Keywords: | Calcium-sensing receptor A549 cells Hypoxia Cell proliferation PI3K/AKT signaling pathway |
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