SLeX对肝癌HepG2细胞迁移和侵袭的影响

目的:探讨唾液酸化路易斯寡糖X(sialyl Lewis X,SLeX)在肝癌HepG2细胞中的表达及其对HepG2细胞迁移能力和侵袭能力的影响。方法:实时荧光定量PCR和Western blot法检测α1,3-岩藻糖基转移酶Ⅶ(α1,3-fucosyltransferase Ⅶ,FUT7)在HepG2细胞和L-02细胞中的表达,Western blot及免疫细胞化学染色检测SLeX在HepG2细胞和L-02细胞中表达,应用Transwell小室检测SLeX单克隆抗体封闭后HepG2细胞侵袭和迁移能力的改变。结果:FUT7和SLeX在HepG2细胞中表达,而在L-02细胞中无表达;0.05、0.5和5 mg/L的SLeX单克隆抗体封闭后,HepG2细胞的迁移率逐渐下降,与对照组相比差异显著(P0.05),侵袭穿膜细胞数明显少于对照组(P0.05);SLeX单克隆抗体封闭组间两两比较迁移率与侵袭细胞数的差异均有统计学意义(P0.05)。结论:SLeX在肝癌HepG2细胞中高表达,与HepG2细胞迁移能力和侵袭能力密切相关。

Effects of SLeX on invasion and migration of HepG2 cells

AIM: To investigate the expression and effects of sialyl Lewis X (SLeX) on the invasion and migration of human hepatocellular carcinoma HepG2 cells. METHODS: The expression of α1,3-fucosyltransferase VII (FUT7) in HepG2 cells and L-02 cells was detected by RT-qPCR and Western blot. The SLeX expression in HepG2 cells and L-02 cells was determined by Western blot and immunocytochemical staining. The invasion and migration abilities of the treated cells were evaluated by Transwell assay. RESULTS: The expression of FUT7 and SLeX in the HepG2 cells, but not in the L-02 cells, was observed. The invasion rates of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L were significantly decreased as compared with control group (P<0.05). The migration ability of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L was also significantly reduced as compared with control group (P<0.05). The invasion rate and migratory cell number were significantly different between any 2 groups in the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L (P<0.05). CONCLUSION: HepG2 cells express SLeX. SLeX is closely related to the migration and invasion abilities of the HepG2 cells.