正天丸对偏头痛大鼠三叉神经节P2X3受体表达的影响

 目的:探讨正天丸对偏头痛大鼠三叉神经节P2X₃受体表达的影响。方法:SD大鼠随机分为对照组、偏头痛组、正天丸组和抑制剂(A-317491)组。给药7 d后,偏头痛组、正天丸组和抑制剂组硝酸甘油(10 mg/kg)皮下注射建立偏头痛大鼠模型,对照组注射等量生理盐水。观察各组大鼠行为学表现,造模4 h后取材,采用免疫荧光、Western blot和实时荧光定量PCR方法观察各组大鼠三叉神经节中P2X₃受体表达的变化。结果:造模后5 min左右各组均出现挠头、爬笼等行为学表现,对照组大鼠仅开始30 min内出现挠头和爬笼现象,无耳红表现,大约2 h 后正天丸组和抑制剂组头痛行为学表现停止,偏头痛组大鼠在造模后3 h左右停止。偏头痛组和对照组相比较,大鼠三叉神经节(trigeminal ganglion,TG)中P2X₃受体蛋白和mRNA表达均明显升高(P<0.05);正天丸组大鼠TG中P2X₃受体蛋白和mRNA表达均明显低于偏头痛组(P<0.01);正天丸组和抑制剂组相比,大鼠TG中P2X₃受体蛋白和mRNA表达无明显差异。结论:正天丸可抑制偏头痛发作时TG中P2X₃受体的过表达,从而发挥抗偏头痛的作用。

Effect of Zhengtian pills on P2X3 receptor expression in trigeminal gan-glion of migraine rats

AIM: To explore the effect of Zhengtian pills on P2X₃ receptor expression in trigeminal ganglion (TG) of migraine rat. METHODS: Sprague-Dawley (SD) rats were randomly divided into control group, migraine group, Zhengtian pills (ZTP) group and A-317491 group. After given corresponding drugs for 7 d, migraine rat model was established by subcutaneously injection of nitroglycerin (10 mg/kg), while the control rats were injected with saline. The beha-vioral manifestations of the rats were observed. The expression of P2X₃ receptor in rat TG was detected by the methods of immunofluorescence, Western blot and rea-time PCR. RESULTS: About 5 min after subcutaneousl injection, the behavioral manifestations such as scratching head and climbing cage were observed. The behavioral manifestations were observed within the first 30 min in control group, but the erythroid ears did not appear. After 2 h of molding, the behavioral manifestations disappeared in ZTP group and A-317491 group, while those in migraine group lasted for 3 h. Immunofluorescence results showed that the expression of P2X₃ receptor in TG was positive in each group. The expression level in migraine group was significantly higher than that in other groups. The P2X₃ receptor protein and mRNA levels in the TG of migraine group were higher than those in control group (P<0.01), while those in ZTP group were lower than those in migraine group (P<0.01). No difference of the P2X₃ receptor expression between ZTP group and A-317491 group was observed. CONCLUSION: Zhengtian pills may effectively alleviate migraine by inhibiting the expression of P2X₃ receptor in TG.