ELMO1在胃癌细胞侵袭和迁移中的作用

目的:探讨吞噬和细胞运动蛋白1(ELMO1)的表达在胃癌细胞侵袭和迁移中的作用和功能。方法:用Western blot和实时荧光定量PCR实验检测5种胃癌细胞和1种人正常胃黏膜上皮细胞ELMO1的蛋白和mRNA表达水平,并筛选出ELMO1表达量高的胃癌细胞株;用细胞转染实验沉默胃癌细胞株的ELMO1;用细胞划痕实验以及Trenswell小室迁移和侵袭实验检测抑制该基因的表达对胃癌细胞侵袭和迁移能力的影响。结果:胃癌细胞中ELMO1的表达量明显高于人正常胃黏膜上皮细胞(P0.01),其中SGC7901细胞的ELMO1表达量最高;在SGC7901细胞中ELMO1-siRNA可显著沉默ELMO1的表达(P0.05);沉默ELMO1可显著降低胃癌细胞侵袭和转移的能力(P0.01)。结论:ELMO1在胃癌细胞中高表达,并可促进胃癌细胞的侵袭和迁移。

Role of ELMO1 in invasion and migration of gastric cancer cells

AIM: To reveal the role and function of engulfment and cell mobility 1 (ELMO1) in the invasion and migration of gastric cancer cells.METHODS: The expression of ELMO1 at protein and mRNA levels was detected in 5 kinds of gastric cancer cells and 1 normal human gastric epithelial cells by Western blot and real-time PCR, and the gastric cancer cells with the highest expression of ELMO1 were screened out. The cell transfection experiment was used to silence ELMO1 expression in the gastric cancer cells, and the effect of ELMO1 silencing on the invasion and migration of the gastric cancer cells was detected by Transwell assay.RESULTS: The expression level of ELMO1 in the gastric cancer cells was significantly higher than that in human normal gastric epithelial cells (P<0.01), and the SGC7901 cells had the highest expression level of ELMO1. ELMO1-siRNA significantly silenced the expression of ELMO1 in the SGC7901 cells (P<0.01). Silencing of ELMO1 expression significantly reduced the invasion and migration abilities of the human gastric can-cer cells (P<0.01).CONCLUSION: ELMO1 is highly expressed in the gastric cancer cells, and promotes the invasion and migration abilities of the gastric cancer cells. ELMO1 may become an effective target for the treatment of invasion and metastasis of gastric cancer.