孜然总黄酮抗实验性胃溃疡作用及机制研究

目的:研究孜然总黄酮对大鼠实验性胃溃疡的影响并探讨其作用机制。方法:选择幽门结扎型、乙醇损伤型及利血平诱导型溃疡模型,以胃液量、胃液pH值、溃疡指数和溃疡抑制率为指标观察孜然总黄酮对实验性胃溃疡的影响,并选用胃泌素(GAS)、前列腺素E2(PGE2)和表皮生长因子(EGF)的含量为指标探讨其作用机制。结果:孜然总黄酮对3种实验模型溃疡有极显著抑制作用(P0.01),且3个模型中低剂量组抑制率均高于高剂量组,但效果不如西咪替丁组。与模型组相比,孜然总黄酮高、低剂量组可明显降低胃液量、游离酸度、总酸度以及总酸排出量(P0.01),且能显著剂量依赖性地提高大鼠血清中PGE2和EGF的含量,降低GAS的含量。结论:孜然总黄酮可能通过调节胃黏液分泌以及PGE2、EGF和GAS的含量对实验性胃溃疡起抑制作用。

Effect of cumin total flavonoids on experimental gastric ulcer and its mechanism

AIM:To discuss the effects of cumin total flavonoids on experimental gastric ulcer and the mechanisms.METHODS:Three rat gastric ulcer models induced by pylorus ligation,ethanol or reserpine were selected.Based on these models,the gastric juice,pH value,ulcer index and suppressive rate were recorded to determine the inhibitory effects of cumin flavonoids on rat gastric ulcers.In addition,the concentrations of gastrin (GAS),prostaglandin E₂(PGE₂) and epidermal growth factor (EGF) were detected to further uncover the mechanisms of these effects.RESULTS:Cumin flavonoids exhibited significant anti-gastric ulcer effects on the three models.Low-dose treatment group (P<0.01) demonstrated higher inhibitory effects than high-dose group (P<0.01),and both low-and high-dose groups had lower effects than cimetidine treatment.Furthermore,cumin flavonoids were able to profoundly decrease the amount of gastric juice,free acidity,total acidity and output of total acid,and enhance the expression of PGE₂ and EGF,but down-regulate the level of GAS in rat serum in a dose-dependent manner.CONCLUSION:Cumin flavonoids might inhibit experimental gastric ulcers by affecting and regulating the gastric juice,PEG₂,EGF and GAS.