白皮杉醇抑制前列腺癌细胞株增殖、迁移与侵袭

目的:观察白皮杉醇对前列腺癌细胞株DU145的细胞活力、迁移与侵袭能力的作用并初步讨论其机制。方法:CCK-8法检测不同浓度白皮杉醇(0、5、10、20、40和80μmol/L)作用不同时间(12、24、36和48 h)对DU145细胞活力的影响;划痕实验与Transwell小室法分别检测白皮杉醇对DU145细胞迁移与侵袭能力的影响;Western blot法检测p-JAK2与p-STAT3的蛋白水平。结果:CCK-8法结果显示白皮杉醇呈浓度依赖性抑制DU145细胞的活力;给予白皮杉醇干预后,细胞迁移数和侵袭数显著减少,p-JAK2与p-STAT3蛋白水平显著下降。结论:白皮杉醇呈浓度依赖性抑制前列腺癌细胞的生长,并具有抑制细胞迁移与侵袭的作用,其机制可能与抑制JAK2/STAT3信号通路有关。

Piceatannol inhibits prostate cancer cell proliferation,migration and invasion

AIM: To investigate the effect of piceatannol on the viability, and the abilities of migration and invasion in the prostate cancer cells.METHODS: DU145 cells were treated with piceatannol at different doses (0, 5, 10, 20, 40 and 80 μmol/L) for different time (12, 24, 36 and 48 h) as indicated. The cell viability was assessed by CCK-8 assay. The migration and invasion abilities of the cells were analyzed by wound healing assay and Transwell assay, respectively. The protein levels of p-JAK2 and p-STAT3 were detected by Western blot.RESULTS: Piceatannol dose-dependently decreased the cell viability. After treatment with piceatannol, the abilities of migration and invasion of the cells were significantly inhibited. Moreover, treatment with piceatannol resulted in marked decreases in the protein levels of p-JAK2 and p-STAT3.CONCLUSION: Piceatannol inhibits the viability, migration and invasion of the prostate cancer cells via regulating the JAK2/STAT3 signaling pathway.