miR-193b在宫颈癌中的表达与功能及其对化疗敏感性的影响

目的:探讨微小RNA-193b(miR-193b)在不同宫颈组织中的表达以及其对顺铂作用于宫颈癌细胞敏感性的影响,并初步探讨其作用机制。方法:实时荧光定量PCR(qPCR)检测不同宫颈组织中miR-193b的表达水平。采用脂质体将miR-193b-inhibitor转染至HeLa细胞,Transwell法、CCK-8法和免疫蛋白印迹法分别检测细胞迁移数、细胞对顺铂的敏感性以及细胞中PTEN、Akt、p-Akt和P-糖蛋白(P-gp)的蛋白水平。结果:qPCR结果显示miR-193b在正常宫颈组织及宫颈糜烂组织中呈低表达,在宫颈上皮内瘤变和宫颈癌组织中的表达明显上升;转染miR-193b-inhibitor后,HeLa细胞迁移数明显减少(P0.05),顺铂对HeLa细胞活力的抑制作用进一步增强(P0.05),细胞中PTEN的蛋白表达明显上调(P0.05),Akt的蛋白表达无明显变化,p-Akt和P-gp的蛋白水平明显下调(P0.05)。结论:miR-193b在宫颈癌组织中呈高表达,沉默miR-193b可增加HeLa细胞对顺铂的敏感性,作用机制可能与PTEN-PI3K/Akt通路有关。

Expression and significance of miR-193b in cervical cancer

AIM: To investigate the expression of microRNA-193b(miR-193b) in the cervical tissues, and further to explore the effect of silencing miR-193b on diamminedichloroplatinum(DDP)-treated HeLa cell viability. METHODS: The expression levels of miR-193b in different cervical tissues were examined by qPCR. After transfection of miR-193b-inhibitor, the cell migration was determined by Transwell assay, the sensitivity of HeLa cells to DDP was measured by MTT assay, the protein levels of phosphate and tension homology deleted on chromsome ten(PTEN), protein kinase B(Akt), p-Akt and p-glycoprotein(P-gp) were determined by Western blot. RESULTS: The mRNA level of miR-193b was significantly increased in the cervical cancer tissues compared with normal cervical tissues(P<0.05). Knockdown of miR-193b obviously inhibited migration and enhanced sensitivity to DDP of HeLa cells(P<0.05). Additionally, after transfection of miR-193b-inhibitor, the expression of PTEN was increased, whereas the protein levels of p-Akt and P-gp were decreased(P<0.05). CONCLUSION: miR-193b is highly expressed in the cervical cancer tissues. Inhibition of miR-193b augments the sensitivity to DDP of HeLa cells, at least in part, through PTEN-PI3K/Akt signaling pathway.