| 川芎嗪与葛根素合用对糖氧剥夺诱导的体外血脑屏障通透性的影响 |
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| 引用本文： | 李金辉,车玲艳,王玉,张宇燕,万海同,杨洁红. 川芎嗪与葛根素合用对糖氧剥夺诱导的体外血脑屏障通透性的影响[J]. 中国中药杂志, 2010, 35(20): 2745-2748 |
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| 作者姓名： | 李金辉 车玲艳 王玉 张宇燕 万海同 杨洁红 |
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| 作者单位： | 浙江中医药大学,心脑血管病研究所,浙江,杭州,310053 |
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| 基金项目： | 国家自然科学基金项目(30873430,30973933) |
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| 摘 要： | ![]()
目的:探讨川芎嗪与葛根素合用对糖氧剥夺诱导的体外血脑屏障(aBBB)通透性的影响。方法:实验分为正常对照组、模型组、川芎嗪组、葛根素组、葛根素与川芎嗪合用组和尼莫地平组6个组。原代培养大鼠脑微血管内皮细胞和星形胶质细胞共培养构建aBBB模型,经糖氧剥夺诱导处理建立aBBB损伤模型,跨内皮电阻抗、γ-谷氨酰转肽酶、碱性磷酸酶及乳酸脱氢酶鉴定aBBB损伤情况,高效液相色谱法测定川芎嗪、葛根素及尼莫地平的含量,并计算在OGD诱导下的aBBB药物透过浓度。结果:与对照组相比,模型组TEER值降低(P0.01),川芎嗪组、川芎嗪与葛根素合用组、尼莫地平组-γGT,AKP升高(P0.01),与川芎嗪与葛根素合用组相比,川芎嗪、葛根素单用AKP升高明显(P0.01),尼莫地平各指标均有显著性差异(P0.05),提示川芎嗪与葛根素合用在可升高TEER,增加-γGT,AKP含量和降低LDH具有协同作用,OGD诱导下aBBB模型的培液中川芎嗪与葛根素合用组各成分透过浓度均较川芎嗪、葛根素单用增加。结论:川芎嗪与葛根素合用较两者单用易透过aBBB,且对aBBB有一定的保护作用,可能与降低OGD诱导下的aBBB通透性有关。
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| 关 键 词： | 川芎嗪 葛根素 体外血脑屏障 通透性 |
| 收稿时间： | 2009-12-31 |
Permeability of blood-brain barrier oxygen-glucose deprivation induced by tetramethylpyrazine-puerarin in vitro |
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| LI Jinhui,CHE Lingyan,WANG Yu,ZHANG Yuyan,WAN Haitong and YANG Jiehong. Permeability of blood-brain barrier oxygen-glucose deprivation induced by tetramethylpyrazine-puerarin in vitro[J]. China Journal of Chinese Materia Medica, 2010, 35(20): 2745-2748 |
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| Authors: | LI Jinhui CHE Lingyan WANG Yu ZHANG Yuyan WAN Haitong YANG Jiehong |
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| Affiliation: | Institute of Cerebro-Cardiovascular Diseases, Zhejiang Chinese Medicine University, Hangzhou 310053, China;Institute of Cerebro-Cardiovascular Diseases, Zhejiang Chinese Medicine University, Hangzhou 310053, China;Institute of Cerebro-Cardiovascular Diseases, Zhejiang Chinese Medicine University, Hangzhou 310053, China;Institute of Cerebro-Cardiovascular Diseases, Zhejiang Chinese Medicine University, Hangzhou 310053, China;Institute of Cerebro-Cardiovascular Diseases, Zhejiang Chinese Medicine University, Hangzhou 310053, China;Institute of Cerebro-Cardiovascular Diseases, Zhejiang Chinese Medicine University, Hangzhou 310053, China |
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| Abstract: | ![]()
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| Keywords: | puerarin tetramethylpyrazine artificial blood brain-barrier permeability |
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