白念珠菌L型研究初报

近年来,关于细菌L型的报道日渐增多,但国内尚未见有真菌L型的正式报道。鉴于系统性念珠菌病血培养阳性率很低,推测是否真菌也存在它的L型。故本文采用酮康唑在高渗培养基上连续诱导白念珠菌C_(1g),体外成功地获取一株部分缺壁的白念珠菌C_(1g)~8,并对其生物特性加以探讨,现将结果报告如下。

Study on L-form induction of C. albicans]

After culturing C. albicans with ketoconazole on high osmosing medium containing thioglycollate, pig serum and sucrose, a cell wall-deficient strain of C. albicans was obtained. We compared its physiologic and biochemical characteristics with those of the parent strain. This cell wall-deficient strain not only changed its morphology but also the chemical components of the cell wall. The cell wall-deficient strain lacked 4 characteristic protein bands and had cell wall mannan decreased to 44% of that of the parent strain. Animal models revealed differences between the toxicities of the two strains: the mean survival time of mice inoculated intravenously with 10(6) deficient strain cells was 23.5 days (70% of the mice died within 30 days), but that of mice injected with 10(6) parent strain cells was 15.8 days (100% of the mice died in 30 days).