针刺对局部脑缺血大鼠大脑皮层基质金属蛋白酶-2表达的影响

目的：观察针刺对局部脑缺血大鼠大脑皮层基质金属蛋白-2（MMP-2）表达的影响。方法：Wistar大鼠24只,分为A、B、C3组各8只,A组大鼠不做任何处理,B、C组建立大鼠大脑中动脉阻塞模型（MCAO）,C组于造模结束30min后针刺大鼠肢体,5h后再针刺1次,以后每天1次,均20min。3组大鼠均采用半定量逆转录聚合酶链反应（RT-PCR）观察大鼠皮层MMP-2 mRNA的表达,免疫组化观察MMP-2蛋白的表达。结果：A组大鼠大脑皮层MMP-2 mRNA和MMP-2蛋白有少量表达,B组增高明显（P〈0.01）;与B组比较,C组MMP-2 mRNA及MMP-2蛋白表达也明显降低（P〈0.01,0.05）。结论：针刺可能通过降低MMP-2表达,减少脑水肿,保护脑组织。

The Effects of Acupuncture on MMP-2 Expression in Cerebral Cortex after Focal Cerebral Ischemia in Rats

Objective: To investigate the effects of acupuncture on the MMP-2 expression in cerebral cortex after permanent focal cerebral ischemia in rats. Methods: Ischemia injury was induced by middle cerebral artery occlusion (MCAO). Acupuncture was administrated for 6 days after MCAO. The expression of MMP-2 mRNA and protein was detected by RT-PCR and immunohistochemistry respectively. Results: Six days after MCAO, the expression levels of MMP-2 mRNA and protein was increased significantly (P<0.01). Application of acupuncture could significantly decrease the mRNA expression of MMP-2 (P<0.01). The protein expression of MMP-2 could also be decreased by the acupuncture (P<0.05). Conclusion: Acupuncture can alleviate the injury of brain by decreasing the expression of MMP-2 in rats with focal cerebral ischemia.