食管小细胞癌凋亡蛋白酶Caspase-3的表达与Survivin Ki67的相关性

目的:探讨凋亡关键蛋白酶Caspase-3在食管小细胞癌中细胞凋亡的病理过程及与凋亡抑制基因Survivin、细胞增殖核抗原Ki67之间的关系。方法:采用免疫组织化学SP法检测Caspase-3、Survivin、Ki67蛋白在食管小细胞癌中的表达。结果:39例食管小细胞癌中Caspase-3蛋白阳性表达率为61.50%,Survivin和Ki67分别为25.60%和46.20%。Caspase-3阳性表达与Survivin呈负相关,P<0.05;与Ki67呈显著负相关,P<0.001。Survivin阳性表达与Ki67呈正相关,P<0.001。结论:1)Caspase-3、Surivivin和Ki67共同参与了食管小细胞癌的凋亡调控过程,可能在食管小细胞癌发生、发展中起重要作用;2)在食管小细胞癌中,凋亡关键蛋白酶表达水平较高,凋亡抑制基因Survivin表达下调,细胞增殖水平相对不高。

Expression of Caspase-3 Correlates with Apoptosis and Proliferation during Human Small Cell Carcinoma of Esophageal Tumorigensis

Objective:To exploring cell apoptosis pathologic process and to analyse expres-sion caspase-3correlates with survivin and Ki67.Methods:Immunohistochemical staining for the paraffin Sections by using the monoclonal antibodies.caspase-3,survivin and Ki67was performed by SP technigue.Results:expression of caspase-3was61.5%and It was inversly correlated with survivin(P<0.05)and Ki67(P<0.001).expression of survivin was positively correlated with Ki67(P<0.001).Conclusions:These results suggest that,like caspase-3,survivin and Ki67play an im portant role in cell apoptosis and pro liferation during human small cell carcinoma of esophageal tumorigenesis.