| 灯盏花颗粒中野黄芩苷的含量测定 |
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| 引用本文： | 陈楚萍,王建壮,周晔. 灯盏花颗粒中野黄芩苷的含量测定[J]. 广东药学院学报, 2010, 26(3): 279-282. DOI: 10.3969/j.issn.1006-8783.2010.03.016 |
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| 作者姓名： | 陈楚萍 王建壮 周晔 |
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| 作者单位： | 广东省医学科学院/广东省人民医院药学部,广东广州,510080;广东药学院药科学院,广东广州,510006 |
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| 摘 要： | 目的建立灯盏花颗粒中野黄芩苷的含量测定方法。方法采用高效液相色谱法,色谱柱：VydacC18色谱柱（150 mm×4.6 mm,4.5μm）,流动相：乙腈-质量分数0.05%磷酸水溶液（体积比17∶83）,检测波长：335 nm,柱温：30℃。结果野黄芩苷在0.022 6-0.141 1μg范围内与峰面积线性关系良好,r=0.999 9,平均回收率为97.56%,RSD=1.38%（n=9）。结论本方法简便、准确、重现性好,可用于灯盏花颗粒中野黄芩苷的含量测定。
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| 关 键 词： | 灯盏花颗粒 野黄芩苷 高效液相色谱法 |
Determination of scutellarin in Erigerontis herba granules |
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| CHEN Chu-ping,WANG Jian-zhuang,ZHOU Ye. Determination of scutellarin in Erigerontis herba granules[J]. Academic Journal of Guangdong College of Pharmacy, 2010, 26(3): 279-282. DOI: 10.3969/j.issn.1006-8783.2010.03.016 |
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| Authors: | CHEN Chu-ping WANG Jian-zhuang ZHOU Ye |
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| Affiliation: | 1.Department of Pharmacy,Guangdong Academy of Medical Sciences/Guangdong General Hospital,Guangzhou,Guangdong 510080,China;2.School of Pharmacy,Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,China) |
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| Abstract: | Objective To establish a method for determining scutellarin in Erigerontis herba granules. Methods C18 column（150 mm ×4.6 mm,4.5 μm）was used at column temperature of 30 ℃.The mobile phase was composed of Acetonitrile-0.05% phosphoric acid solution（17∶83）.And the detection wavelength was set at 335 nm.Results The calibration curve was linear in the range of 0.022 6-0.141 1 μg（r=0.999 9）.The average recovery of scutellarin from granules was 97.56% with RSD of 1.38%（n=9）.Conclusion The method is reliable,accurate and can be used in the quantitative analysis of scutellarin in Erigerontis herba granules. |
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| Keywords: | Erigerontis herba granules scutellarin HPLC |
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