肺金生方对Lewis肺癌小鼠VEGF-C、 nm23表达的影响

目的:探讨肺金生方对Lewis肺癌小鼠VEGF-C、nm23表达的影响。方法:取60只C57BL/6小鼠,常规接种Lewis肺癌瘤株,随机分为生理盐水组、环磷酰胺(CTX)组、中药低剂量组、中药中剂量组、中药高剂量组、CTX加中药组,每组10只。各组给予相应药物灌胃14天后处死,取肺组织观察肺转移数,取肿瘤组织甲醛固定后免疫组化检测VEGF-C、nm23表达。结果:肺金生方中、高剂量对Lewis肺癌小鼠自发肺转移有抑制作用(P<0.01),中药肺金生方各剂量组及CTX、CTX加中药组均能降低VEGF-C表达(P<0.01、P<0.05),而中药中、高剂量组、CTX加中药组均能提高nm23的表达(P<0.01),中药中、高剂量组nm23表达比CTX加中药中剂量组更高(P<0.05)。结论:肺金生方抑制Lewis肺癌小鼠自发肺转移可能与其降低VEGF-C表达和提高nm23的表达有关。

Effects of Feijinsheng Decoction on the Expressions of VEGF-C and nm23 in Mice with Lewis Lung Car? cinoma

Objective:To evaluate the effect of Fei-jin-sheng Decoction on VEGF-C,nm23 expression in Lew is lung cancer mice.Method:Sixty C57BL/6 mice inoculated with Lewis lung cancer cell line were randomly di vided into 6 groups:normal saline group,cyclophosphamide(CTX) group,Feijinsheng decoction groups(low-,medi um-,and high-dose subgroups),and CTX combined with medium-dose Feijinsheng group,10 mice in each group.The corresponding drugs were intragastrically given to the groups once a day for 14 days and then the mice were executed for observation of lung metastasis and detection of the expressions of VEGF-C and nm23 in the lung tissues by immunohistochemistry.Results:The medium-dose and high-dose Feijinsheng decoction groups had less spontaneous pulmonary metastasis(P<0.01).All groups except the normal saline group reduced VEGF-C expression(P<0.01 or P<0.05).The medium-dose and high-dose of Fei-jin-sheng decoction groups and CTX combine with medium-dose Feijinsheng group elevated the expression of nm23(P<0.01),and the expression lev els in the medium-dose and high-dose of Fei-jin-sheng decoction groups were higher than that in the combina tion group(P<0.05).Conclusion:Fei-jin-sheng decoction can inhibit the spontaneous lung metastasis of Lewis lung cancer in mice by reducing VEGF-C while elevating nm23.