曲马多预处理对氯胺酮抗抑郁作用的影响

目的:观察曲马多预处理对氯胺酮抗抑郁作用的影响。方法:32只Wistar大鼠随机分为生理盐水组(S组)、曲马多组(T组)、氯胺酮组(K组)、曲马多+氯胺酮组(T+K组)。药物预处理前1天大鼠强迫游泳15 min;预处理当天各组分别腹腔注射1 mL容积的生理盐水、曲马多5 mg.kg-1、生理盐水、曲马多5 mg.kg-1;30 min后,各组分别注射生理盐水、生理盐水、氯胺酮10 mg.kg-1、氯胺酮10 mg.kg-1;再经30 min后,行强迫游泳试验并记录其不动时间,取海马组织测定脑源性神经营养因子(BDNF)及酪氨酸受体激酶B(TrkB)的含量。结果:与S组相比,K组、T+K组强迫游泳试验不动时间减少,海马BDNF及TrkB表达增加(P<0.05);与K组相比,T+K组强迫游泳试验不动时间减少,海马BDNF及TrkB表达增加(P<0.05)。结论:曲马多预处理能增强氯胺酮的抗抑郁作用,这可能与大鼠海马BDNF及TrkB表达上调有关。

Effects of Tramadol Pretreatment on Ketamine-induced Antidepressant Action

Objective: To observe the effects of tramadol pretreatment on ketamine-induced antidepressant action. Methods: Thirty-two Wistar rats were randomly divided into saline group (S group), tramadol group (T group), ketamine group (K group) and tramadol+ketamine group (T+K group). One day before drug pretreatment, rats were insulted in the forced swimming test (FST) for 15 min. At the day of drug pretreatment, rats were intraperitoneally injected with 1 ml of saline, tramadol 5 mg·kg^-1, saline and tramadol 5 mg·kg^-1, respectively; Thirty minutes later, rats were intraperitoneally injected with saline, saline, ketamine 10 mg·kg^-1 and ketamine 10 mg·kg^-1, respectively. Another 30 min later, FST was conducted and the immobility time was recorded. Rat hippocampus was harvested and the levels of brain-derived neurotrophic factor (BDNF) and tyrosine receptor kinases B(TrkB) were detected. Results: Compared with S group, the immobility time of rats receiving FST decreased and the expression of hippocampal BDNF and TrkB increased in K and T+K groups (P<0.05). Compared with K group, the immobility time decreased and the expression of hippocampal BDNF and TrkB increased in T+K group. Conclusion: Tramadol pretreatment can reinforce the antidepressant action of ketamine, which may be attributed to the increase in the expression of hippocampal BDNF and TrkB.