| 胰岛素对大鼠平滑肌细胞表达MMP-9、TIMP-1及内皮细胞表达sES的影响 |
| |
| 引用本文： | 葛晨亮,刘厂辉,唐振旺,吴洁,苏华,王刚,占凡,谢楠,陈仲. 胰岛素对大鼠平滑肌细胞表达MMP-9、TIMP-1及内皮细胞表达sES的影响[J]. 中国动脉硬化杂志, 2016, 24(2): 145-149 |
| |
| 作者姓名： | 葛晨亮 刘厂辉 唐振旺 吴洁 苏华 王刚 占凡 谢楠 陈仲 |
| |
| 作者单位： | 南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001,南华大学附属第一医院心血管内科,湖南省衡阳市 421001 |
| |
| 基金项目： | 湖南省卫生厅科研基金(B2012-049) |
| |
| 摘 要： | ![]()
目的探讨胰岛素对SD大鼠平滑肌细胞表达基质金属蛋白酶9(MMP-9)、组织抑制因子1(TIMP-1)以及内皮细胞表达可溶性E-选择素(s ES)的影响。方法原代培养SD大鼠颈内动脉平滑肌细胞及主动脉内皮细胞,用不同剂量的胰岛素作用后,以酶联免疫吸附实验(ELISA)分别测定上清液MMP-9、TIMP-1及s ES的水平。结果与对照组(无胰岛素)相比,胰岛素组MMP-9及s ES水平显著增高,TIMP-1则显著降低(P0.05)。经Pearson相关分析显示,胰岛素浓度与MMP-9(r=0.815,P0.001)及s ES(r=0.841,P0.001)呈正相关,与组织抑制因子1(r=-0.740,P0.001)呈负相关。结论胰岛素促进SD大鼠平滑肌细胞MMP-9及内皮细胞s ES表达,抑制平滑肌细胞TIMP-1表达,并呈剂量依赖性。
|
| 关 键 词： | 胰岛素 基质金属蛋白酶9 组织抑制因子1 E-选择素 |
| 收稿时间： | 2015-08-07 |
| 修稿时间： | 2015-11-10 |
Effect of Insulin on the Expression of MMP9 and TIMP-1 Released from Smooth Muscle Cells and E-selectin Released from Endothelial Cells in SD Rats |
| |
| GE Chen-Liang,LIU Chang-Hui,TANG Zhen-Wang,WU Jie,SU Hu,WANG Gang,ZHAN Fan,XIE Nan and CHEN Zhong. Effect of Insulin on the Expression of MMP9 and TIMP-1 Released from Smooth Muscle Cells and E-selectin Released from Endothelial Cells in SD Rats[J]. Chinese Journal of Arteriosclerosis, 2016, 24(2): 145-149 |
| |
| Authors: | GE Chen-Liang LIU Chang-Hui TANG Zhen-Wang WU Jie SU Hu WANG Gang ZHAN Fan XIE Nan CHEN Zhong |
| |
| Affiliation: | Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China,Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China and Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China |
| |
| Abstract: | ![]()
|
| |
| Keywords: | Insulin Metalloproteinase-9 Tissue Inhibitor of Metalloproteinase-1 E-selectin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国动脉硬化杂志》浏览原始摘要信息 |
|
点击此处可从《中国动脉硬化杂志》下载全文 |
|
