Survivin与MHC在非小细胞肺癌中的表达及临床意义

目的 研究非小细胞肺癌(NSCLC)组织和正常组织中Survivin和主要组织相容性复合体(MnC)的表达情况,以及相互之间的关系.方法 应用流式细胞仪定量检测4JD例NSCLC组织、15例正常肺组织中Survivin蛋白及MHC的表达情况.结果 NSCLC中Survivin的阳性表达率在Ⅰ、Ⅱ、Ⅲ期患者中分别是28.57%、30.77%、80.00%,Ⅰ期与Ⅲ期之间及Ⅱ期与Ⅲ期差异有统计学意义(P=0.013,P=0.005);Ⅰ+Ⅱ期与Ⅲ期之间差异有统计学意义(P=0.01);淋巴结转移阳性组与阴性组之间差异有统计学意义(P=0.030);NSCLC中MHC-Ⅰ的失表达率在高分化组、中分化组、低分化组中分别是37.50%、42.10%、84.62%,低分化组明显低于高分化组及中分化组(P=0.026,P=0.016);NSCLC中MHC-Ⅱ的阳性表达率与NSCLC病理类型有关(P=0.005),鳞癌及非鳞非腺癌中MHC-Ⅱ表达均低于腺癌(P=0.002,P=0.04);NSCLC中Survivin表达与MHC-Ⅰ、MHC-Ⅱ表达无明显相关性.结论 Survivin与MHC在NSCLC的发生发展中有重要作用,联合检测有助于NSCLC患者预后的判断.

Expression and clinical significance of Survivin and MHC in non-small cell lung cancer

Objective To explore the expression of Survivin and MHC in non-small cell lung cancer (NSCLC), and their interaction.Methods A total of 40 patients with histologically diagnosed NSCLC were enrolled in this study.Control samples were consisted of normal lung tissues from 15 patients.Expression of Survivin and MHC were detected by flow cytometry in 40 non-small cell lung cancer tissues and 15 normal lung tissues.Results The positive rates of Survivin protein expression in NSCLC tissues classified stages Ⅰ,Ⅱ and Ⅲ were 28.57% ,30.77% and 80.00% respectively.There was a correlation between Survivin protein expres-sion and stages of NSCLC.Survivin protein expression was detected in 19 of 29 patients with lymph node metas-tasis, and 3 of 11 patients with no metastasis.There was a statistically significant difference between the two groups (P<0.030).The loss expression rates of MHC-Ⅰ in NSCLC tissues of low grade,intermediate grade and high grade were 84.62% ,42.10% and 37.50% respectively.There was a correlation in expression be-tween MHC-Ⅰ and tumor grade.The positive rates of MHC-Ⅱ expression in NSCLC tissues was related to pa-thology type (P=0.005).The expression in squamous cancer and non-squamous non-adno cancer was lower than that of adenocarcinoma (P=0.002, P=0.04).There was no obvious correlation between the expression of Survivin and MHC-Ⅰ,MHC-Ⅱ in NSCLC.Conclution The expression of Survivin and MHC could be in-volved in the pathogenesis and development of NSCLC, and the combined detection will predict the prognosis of the patients with NSCLC.