细胞因子诱导的自体杀伤细胞回输治疗慢性乙型肝炎患者树突状细胞功能的研究

目的了解细胞因子诱导的自体杀伤（CIK）细胞回输治疗是否能恢复慢性乙型肝炎（CHB）患者树突状细胞（DC）的功能。方法12例CHB患者应用CIK细胞回输治疗，入组前6个月至CIK细胞治疗随访期间，患者未接受任何抗病毒及免疫调节治疗。观察24周后患者髓样树突状细胞（mDC）、浆细胞样树突状细胞（pDC）比例和功能变化，并对其变化结果进行分析。结果应用CIK细胞回输治疗24周后，病毒载量下降≥2个log或转阴的患者外周血mDC、pDC功能明显提高。病毒学应答患者（n=6）病毒载量下降水平与外周血DC功能的恢复密切相关。结论CHB患者接受CIK细胞回输治疗可以提高外周血mDC和pDC功能。

Research of the function of dendritic cell subsets in chronic hepatitis B patients after retransfusion treatment with autologous CIK cells

Objective To investigate the function of circulating mDC and pDC subsets in chronic hepatitis B(CHB) patients after retransfusion treatment with autologous CIK cells. Methods Autologous CIK cells obtained from 12 CHB patients were retransfused to the patients themselves, who didn't receive antiviral therapy nor immunomodulatory treatment during six months before enrollment for CIK cells treatment and follow-up period. The changes in the frequency and function of mDCs and pDCs in CHB patients were observed and analyzed at week 24. Results At week 24 after retransfusion treatment with autologous CIK cells, the function of mDC and pDC significantly increased in virologic responders with either negativity or two log decrease of serum HBV DNA load(n=6). There was a close correlation of restoration of DC subsets with the decrease in serum HBV load. Conclusion Retransfusion treatment with autologous CIK cells can improve the functin of circulating mDC and pDC in CHB patients.