| 过氧化氢-乙醚法制备脱蛋白骨的理化性质研究 |
| |
| 引用本文： | 曹凯,舒勇,韩智敏,范伟,安洪,蒋电明,段满生,顾玉荣. 过氧化氢-乙醚法制备脱蛋白骨的理化性质研究[J]. 江西医学院学报, 2008, 48(1): 5-8,F0003 |
| |
| 作者姓名： | 曹凯 舒勇 韩智敏 范伟 安洪 蒋电明 段满生 顾玉荣 |
| |
| 作者单位： | 曹凯（南昌大学,第一附属医院骨科,南昌,330006）;舒勇（南昌大学,第一附属医院骨科,南昌,330006）;韩智敏（南昌大学,第一附属医院骨科,南昌,330006）;范伟（重庆医科大学附属第一医院骨科,重庆,400016）;安洪（重庆医科大学附属第一医院骨科,重庆,400016）;蒋电明（重庆医科大学附属第一医院骨科,重庆,400016）;段满生（南昌大学,第一附属医院骨科,南昌,330006）;顾玉荣（南昌大学,第二附属医院骨科,南昌,330006） |
| |
| 摘 要： | 目的探讨过氧化氢-乙醚法制备脱蛋白骨（deproteinized bone，DPB）的理化性质。方法采用过氧化氢-乙醚法将大白兔干骺端松质骨脱脂、脱蛋白制作成生物衍生骨材料——DPB。倒置相差显微镜、体视学显微镜、扫描电子显微镜观察DPB材料外表面、孔隙内表面结构、孔径大小和孔间交通情况。pH计检测仪检测DPB材料的培养液pH值。光学显微镜观察微生物生长情况。微量凯氏定氮法检测DPB材料中蛋白质含量。结果倒置相差显微镜、体视学显微镜、扫描电子显微镜观察结果显示，DPB材料具有原骨组织的三维多孔一网架结构系统。计算机图像分析系统结果显示，孔隙率为（83．26±5．35）％；孔径最大径为（315．11±17．51）μm，最小径为（109．37±11．33）μm。浸泡DPB材料组培养液pH为7．383±0．015，空白对照组培养液pH为7．380±0．020。光学显微镜下观察结果显示，DPB材料的培养液未发现细菌或霉菌生长。DPB蛋白质含量为（20．4±1.2）％。结论由松质骨来源制作的DPB是一种较理想的组织工程骨生物支架材料。
|
| 关 键 词： | 脱蛋白骨 理化性质 过氧化氢 乙醚 |
| 文章编号： | 1000-2294（2008）01-0005-04 |
| 收稿时间： | 2007-05-23 |
| 修稿时间： | 2007-05-23 |
The Physical and Chemical Character of Deproteinized Bone by Hydrogen Dioxide and Diethyl Ether |
| |
| CAO Kaia,SHU Yonga,HAN Zhi-mina,FAN Wei,AN Hong,JIANG Dian-ming,DUAN Man-shenga,GU Yu-rongb. The Physical and Chemical Character of Deproteinized Bone by Hydrogen Dioxide and Diethyl Ether[J]. Acta Academiae Medicinae Jiangxi, 2008, 48(1): 5-8,F0003 |
| |
| Authors: | CAO Kaia SHU Yonga HAN Zhi-mina FAN Wei AN Hong JIANG Dian-ming DUAN Man-shenga GU Yu-rongb |
| |
| Affiliation: | CAO Kai1a,SHU Yong1a,HAN Zhi-min1a,FAN Wei2,AN Hong2,JIANG Dian-ming2,DUAN Man-sheng1a,GU Yu-rong1b(1a.Department of Othorpedics,First Affiliated Hospital,1b.Department of Othorpedics,Second Affiliated Hospital,Nanchang University,Nanchang 330006,China,2.Department of Othorpedics,the First Affiliated Hospital ofChongqing University of Medical Sciences,Chongqing 400016,China) |
| |
| Abstract: | Objective To investigate the physical and chemical characters of deproteinized bone(DPB)processed with hydrogen dioxide and diethyl ether.Methods Metaphysic cancellous bone collected from adult white rabbit was digested with hydrogen peroxide and diethyl ether which converted the cancellous bone into DPB,a kind of biological derivative scaffold.The morphous of external and internal surface,porus diameter as well as transconnection of DPB was observed under inverted phase contrast microscope,stereomicroscope... |
| |
| Keywords: | deproteinized bone physical and chemical characters hydrogen peroxide diethyl ether |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
